Fate of Ingested Immunoglobulin G in the Mosquito Aedes Aegypti (Diptera: Culicidae)

免疫球蛋白G在埃及斑蚊體內之變化

陳維鈞(Wei-June Chen) ； 陳瑩霖(Eng-Rin Chen)

《The Kaohsiung Journal of Medical Sciences》 6卷11期 (1990/11) Pp. 606-613

https://doi.org/10.6452/KJMS.199011.0606

摘要

The amount of bloodmeals ingested by Aedes aegypti was estimated to be 3.00± 1.05 mg (2.84± 0.99 i1) in average. The feeding time of a mosquito was not necessarily associated with the quantity of the bloodmeal. Nevertheless, the number of egg laid after bloodfeeding was apparently dependent on the volume of ingested bloodmeals. The change of immunoglobulin G (IgG) in the ingested bloodmeals of Aedes aegypti was also evaluated by ELISA.
To detect the survival of ingested IgG, 5-day-old adult female mosquitoes were allowed to feed on a human arm. After completion of feeding, 3 engorged mosquitoes were selected on the basis of 6-hour intervals until the 54th hour post ingestion. Selected specimens were deprived of their legs and wings, homgenized and centrifuged. The supernatants were collected for ELISA procedure. The control groups included the blank (PBS) and unfed mosqutioes.
All 0D405 values representing the amount of IgG in the ingested bloodmeal were evaluated as 1.702±0.017, 0.469±0.046, 0.414±0.084, 0.231±0.012, 0.180±0.025, 0.172±0.006, 0.163±0.012, 0.173±0.007, 0.168±0.012, and 0.169±0.014, respectively for each interval. A blood sample taken from the same person fed to mosqutioes was evaluated to be 1.716±0.012. The 0D405 values of the blank and the unfed group were 0.156±0.019 and 0.159±0.009, respectively.
The result showed that IgG ingested by Aedes aegypti may be digested vigorously wighin 6 hours. It also showed that OD405 values in all groups which were harvested later than 24 hrs post-ingestion were not significantly different (p＞O.O5). The titer of IgG became undetectable since this time. Presumably, IgG in the ingested bloodmeal could have been completely digested within 24 hours.
It was assumed that the antibodies against a specific pathogen, such as dengue virus, will neutralize the ingested antigen if the next blood-feeding occurs within 6 hrs. In contrast, the antibodies would disappear, at least functionally, if the second blood-feeding is taken place later than 24 hrs after the first one.

並列摘要

The amount of bloodmeals ingested by Aedes aegypti was estimated to be 3.00± 1.05 mg (2.84± 0.99 i1) in average. The feeding time of a mosquito was not necessarily associated with the quantity of the bloodmeal. Nevertheless, the number of egg laid after bloodfeeding was apparently dependent on the volume of ingested bloodmeals. The change of immunoglobulin G (IgG) in the ingested bloodmeals of Aedes aegypti was also evaluated by ELISA.
To detect the survival of ingested IgG, 5-day-old adult female mosquitoes were allowed to feed on a human arm. After completion of feeding, 3 engorged mosquitoes were selected on the basis of 6-hour intervals until the 54th hour post ingestion. Selected specimens were deprived of their legs and wings, homgenized and centrifuged. The supernatants were collected for ELISA procedure. The control groups included the blank (PBS) and unfed mosqutioes.
All 0D405 values representing the amount of IgG in the ingested bloodmeal were evaluated as 1.702±0.017, 0.469±0.046, 0.414±0.084, 0.231±0.012, 0.180±0.025, 0.172±0.006, 0.163±0.012, 0.173±0.007, 0.168±0.012, and 0.169±0.014, respectively for each interval. A blood sample taken from the same person fed to mosqutioes was evaluated to be 1.716±0.012. The 0D405 values of the blank and the unfed group were 0.156±0.019 and 0.159±0.009, respectively.
The result showed that IgG ingested by Aedes aegypti may be digested vigorously wighin 6 hours. It also showed that OD405 values in all groups which were harvested later than 24 hrs post-ingestion were not significantly different (p＞O.O5). The titer of IgG became undetectable since this time. Presumably, IgG in the ingested bloodmeal could have been completely digested within 24 hours.
It was assumed that the antibodies against a specific pathogen, such as dengue virus, will neutralize the ingested antigen if the next blood-feeding occurs within 6 hrs. In contrast, the antibodies would disappear, at least functionally, if the second blood-feeding is taken place later than 24 hrs after the first one.

並列關鍵字

blood meal ； ELISA ； Aedesaegypti