Variations in Complement Activation of Brugia Patei, B. Malayi and B. Pahangi Microfilariae

Brugia patei, B. malayi及B. pahangi微仔蟲補體活化的差異

U. R. Rao ； A. C. Vickery ； J. K. Nayar ； B. H. Kwa

《The Kaohsiung Journal of Medical Sciences》 7卷4期 (1991/04) Pp. 160-167

https://doi.org/10.6452/KJMS.199104.0160

摘要

Brugia malayi和B. pahangi帶鞘的微仔蟲可以活化BALB／C（nu／＋）和B／C（nu／nu）的補體，而且可藉燭光抗體法偵測到結合在帶鞘微仔蟲表面的Ca，活化的補體可促進吞噬細胞及嗜中性白血球吸附並毒殺微仔蟲，而這些作用也發生在B. patei及B. malayi脫鞘微子蟲，若將補體經加熱或zymosan處狂或加入抗鼠Ca則這些作用均消失。EDTA可使補體失去促進細胞吸附B. malayi帶鞘及脫鞘微仔蟲的能力，而EGTA及EDTA則可使補體失去促進細胞吸附脫鞘B. patei及帶鞘B. pahangi微仔蟲的能力，顯示活化補體的途徑有所不同。nu／＋或nu／nu腹腔滲出液內的書噬細胞及嗜中性白血球對微仔蟲吸附及毒殺的能力相同，BALB/C鼠腹腔的洗出液內僅發現B. patei的微仔蟲而且沒有細胞吸附及毒殺現象。

並列摘要

The complement of fresh normal heterozygotic BALB/C (nu/+) and nude (nu/nu) mice was activated by sheathed microfilariae (Mf) of Brugia malayi and B pahangi but not by B patei. Mouse C3 was detected by immunofluorescence on the sheathed surface of B. malayi and B. pahangi Mf but not on B. patei Mf. The bound complement promoted macrophage and neutrophil mediated adherence and cytotoxicity to these Mf. However, BALB/C nu/nu mouse macrophages and neutrophils adhered to exsheathed Mf of B. patei and killed them in the presence of fresh normal mouse serum (NMS). C3 molecules were detected on the cuticle of exsheathed Mf of B. patei and B. malayi. Fresh NMS (nu/+ or nu/nu) depleted of complement factors by heating or by zymosan or anti-mouse C3, failed to promote cell adherence to these parasites. EDTA but not EGTA abolished the adherence activity to sheathed and exsheathed Mf of B. malayi, whereas both EGTA and EDTA abolished the adherence activity to exsheathed Mf of B. patei and sheathed Mf of B. pahangi suggesting the different pathways of complement activation by these closely related species. Macrophages and neutrophils from peritoneal exudate cells of nu/+ or nu/nu were equally poyrny in sfhrtrnvr snf killinh of Mf. Transfused B. patei but not B. malayi or B. pahangi Mf were recovered from the peritoneal cavity of BALB/C mice with no cell adherence and cytotoxicity.

並列關鍵字

complement ； microfilariae ； cytotoxicity