Thin - Layer Chromatographic Detection of Glucocorticoids

薄層層析法對口服用糖類皮質類固醇之鑑別試驗

吳秀梅(Shou-Mei Wu) ； 陳素惠(Su-Hwei Chen) ； 吳信隆(Hsin-Lung Wu)

《The Kaohsiung Journal of Medical Sciences》 7卷11期 (1991/11) Pp. 545-549

https://doi.org/10.6452/KJMS.199111.0545

摘要

利用薄層層析法配合衍生反應對口服用八種糖類板質類固醇建立簡單檢索方法。本法以silica為固定相，氯依－二氧六圜－甲醇（15：4：1，v / v）為展開溶媒，嘗試了triamcinolone, prednisolone, 6α-methylprednisolone, betamethasone, dexamethasone, hydrocortisone, prednisone及cortisone等糖類板質類固醇之分離方法，除了betamethasone及dexamethasone二種差向立體異搆物（epimer）外，其餘皆能順利分離。經以(1S)-(-)-camphanic chloride為不對稱衍生試劑，將betamethasone及dexamethasone加以衍生，所以衍生物可理想分離。本法對各種檢驗體中糖類板質類固醇之應用分析正順利推展中。

並列摘要

An adsorption thin-layer chromatography (TLC) is described for the detection of glucocorticoids including triamcinolone, perdnisolone, 6α-methylprednisolone, betamethasone, dexamethasone, hydrocortisone, prednisone and cortisone. Separation of the glucocorticoids was performed on a silica stationary phase with chloroform-dioxane-methanol (15: 4: 1, v/v) as a mobile phase. The complete resolution of the epimers of betamethasone and dexamethasone was attained by derivatization of them with (1S)-(-)-camphanic chloride using triethylamine as a catalyst. The application of the method to the analysis of glucocorticoids in various samples is being smoothly developed.

並列關鍵字

TLC ； detection ； glucocorticoids