Nucleolar Organizer Regions in Small Cell Carcinoma of Lung

肺部小細胞癌的核仁組合體區

林茂生(Maw-Sheng Lin) ； 黃吉志(Jhi-Jhu Hwang) ； 鍾飲文(Inn-Wen Chong) ； 蔡米山(Mee-Sun Tsai)

《The Kaohsiung Journal of Medical Sciences》 10卷4期 (1994/04) Pp. 182-185

https://doi.org/10.6452/KJMS.199404.0182

摘要

Agyrophilic staining of nucleolar organizer regions (NOR) has been used to differentiate between cells of small cell carcinoma and lymphocytes in tissue speciemens. We used cytologic smears which were previously Papanicolaou-destained to study the one-step agyrophilic staining technique for nucleolar organizer regions (AgNOR) in cell of small cell carcinoma and lymphocyte. The purposes of this study were to assess the feasibility and usefulness of AgNOR staining in diagnostic cytology and to try to set up a procedure that could be used on prestained smears for retrospective study. While the NOR of each lymphocyte appeared to be one round dot after AgNOR staining, the NOR of cell of small cell carcinoma showed dots, slightly variable in size and shape. The mean number of NOR was significantly higher (p ＜0.01) in cells of small cell carcinoma (4.7±0.7) than in lymphocytes (1.4±0.4). In conclusion, AgNOR staining was demonstrated to be a useful method to differertiate between cells of small cell carcinoma and lymphocyte in Papanicolaou-destained smears.

並列摘要

Agyrophilic staining of nucleolar organizer regions (NOR) has been used to differentiate between cells of small cell carcinoma and lymphocytes in tissue speciemens. We used cytologic smears which were previously Papanicolaou-destained to study the one-step agyrophilic staining technique for nucleolar organizer regions (AgNOR) in cell of small cell carcinoma and lymphocyte. The purposes of this study were to assess the feasibility and usefulness of AgNOR staining in diagnostic cytology and to try to set up a procedure that could be used on prestained smears for retrospective study. While the NOR of each lymphocyte appeared to be one round dot after AgNOR staining, the NOR of cell of small cell carcinoma showed dots, slightly variable in size and shape. The mean number of NOR was significantly higher (p ＜0.01) in cells of small cell carcinoma (4.7±0.7) than in lymphocytes (1.4±0.4). In conclusion, AgNOR staining was demonstrated to be a useful method to differertiate between cells of small cell carcinoma and lymphocyte in Papanicolaou-destained smears.

並列關鍵字

nucleolar organizer region ； small cell carcinoma ； Papanicolaou stain