- 期刊
In Vitro Production of Interleukin-6 by Human Gingival, Normal Buccal Mucosa; and Oral Submucous Fibrosis Fibroblasts Treated with Betel-Nut Alkaloids
人類健康牙齦、健康頰黏膜以及黏膜下纖維化症頰黏膜等造纖維細胞經檳榔生物鹼作用後介白質-6分泌之研究
摘要
本研究之目的，在評估檳榔生物鹼（ are - coline 與 arecaidine ）作用於健康牙齦、健康頰粕膜以及口腔粕膜下纖維化症（ OSF ）之頰粕膜等三種造纖維細胞後，對細胞增殖與介白質一 6 ( IL-6 ）合成分泌之影響。實驗時，分別培養此三種組織細胞，在經過四種不同濃度 ( 0 . 1 , l , 10 , 100 ” g / ml ）之檳榔生物鹼作用 45 小時後，測量細胞增殖情形以及條件性培養基中 IL-6 之濃度。結果顯示，在細胞增殖方面，除了當 arecoline 濃度為100μg / ml 時，有抑制細胞生長之現象外， arecoline 與 arecaid - ine 對三種造纖維細胞之增殖影響，均呈現隨著濃度增加而增加。至於在未經填加檳榔生物鹼之條件性培養基中 IL-6 濃度顯示，健康牙齦細胞分泌最高，健康頰枯膜次之， OSF 頰牯膜分泌最低；而培養基中填加小牛血清有助於 IL-6 分泌。此外，在填加檳榔生物鹼之條件性培養基中， IL-6 濃度數據若以平均值士標準偏差來表示，則各種組織細胞之實驗韋與對照韋之問，除了 arecoline 在 100μg / ml 時， IL-6 明顯減少以外，其餘各韋問均沒有統計上之差別意義。然而，若以細胞株單獨變化而言，在健康頰粕膜六株造纖維細胞中，有兩株經檳榔生物鹼作用後珍一 6 生成降低。相反地，部份 OSF 與健康牙齦細胞株，經檳榔生物鹼作用後， IL-6 生成濃度則有些微增加。由上述結果可得以下結論，檳榔生物鹼（ arecoline 與 ar - eeaidin 。）會促進細胞增殖，並且可以影響造纖維細胞合成分泌 IL-6 。此外 IL-6 在 oSF 之致病因上可能占有某一特定角色。
關鍵字
無資料並列摘要
This study aimed to assess the possibility of a direct effect of betel-nut alkaloids arecoline and arecaidine on cell proliferation and interleukin-6 (IL-6) production by cultured fibroblasts from human normal gingiva, buccal mucosa and oral submucous fibrosis (OSF) buccal mucosa in vitro. Confluent monolayers of fibroblasts were incubated with or without alkaloids in the presence of 10% fetal calf serum for 48 h at 37 ‘C in 5% CO2 and air. At the end of the culture period, supernatants were collected and assayed for IL-6 level. The cell proliferation was monitored by determining 5-bromo-2’ - deoxy-uridine (BrdU) incorporated into cellular DNA. Except for the fact that arecoline inhibited cell growth at 100j.tg/ml, arecoline and arecaidine had similar dose-dependent stimulant effects on the proliferation of these three groups fibroblasts. Concentrations of IL-6 in the control culture supernatants were greatest in healthy gingival fibroblasts, followed by normal buccal mucosa and OSF. Also, the presence of fetal calf serum could stimulate IL-6 release. Except for arecoline at lOOjtg/ml, there were no significant differences in IL-6 levels between treated and control cultures of the same group when the data were expressed with mean ± S.D.. However, two of six individuals’ normal buccal mucosa fibroblasts significantly released less IL-6, and some cases of OSF and healthy gingiva exhibited slightly higher levels of IL-6 when cells were exposed to arecoline or arecaidine in cultures. Such findings suggest that arecoline and arecaidine can enhance cell proliferation and affect fibroblasts to synthesize IL-6. Furthermore, IL-6 may be a contributing molecular factor in the pathological features noted in OSF.