- 期刊
Detection of Iron (III) Chelate Separated by Reversed-Phase High-Performance Liquid Chromatography
利用高效率液體層析儀分析鑑定鐵（III）錯合物
摘要
本研究係探討逆相高效率液體層析儀分析鑑定磁振造影對比劑iron（III）-N,N’-trimethyle-nebis [ 2-（2-hydroxyl-5-ethyl-phenyl）glycine]（Fe ( TM-5-ethyl-PHPG）-）之立體異構物，分別改變移動相之pH值，不同離子配對試劑（ion-pairing agents）及其濃度，觀察其對鐵（III）錯合物之立體異構物及游離配位子分離之容量因子（capacity factor）之影響，進而找出分離鐵（III）錯合物立體異構物及游離配位子最佳分離條件，同時將高效率液體層析儀接離子交換管柱進一步分析鐵（III）錯合物中游離鐵離子之濃度，此方法可做為磁振造影對比劑分析及鑑定之依據。
關鍵字
無資料並列摘要
A reversed-phase high-performance liquid chromatographic method for the analysis of iron(III)-N, N’-trimethylenebis[2-(2-hydroxyl-5-ethyl-phenyl)glycine] (Fe(TM-5-ethyl- PHPG)-), which is potentially used as effective hepatobiliary magnetic resonance imaging (MRI) contrast agent, has been developed in this study. The effect of mobile phase, such as the varied pH values, ion-pairing agents and concentration of the ion-pairing agent on the capacity factor of the diastereomers and free ligand, were investigated to improve the separation and peak shape. HPLC in connection with a ion exchange column and a UV/VIS detector can be used to determine metal ion dissociated from this complex. This protocol can be successfully used in the quality assay of the synthesized potential MRI contrast agent of [Fe(TM-5-ethyl-PHPG)-].
延伸閱讀
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