Human Prolactinomas in Cell Culture: Structure - Function Correlation and Indication of Early Relapse

細胞培養中腦垂促乳素腺瘤構造功能間之相關性及早期再發之研究

王致仁(Chi-Jen Wang) ； 洪純隆(Shen-Long Howng)

《The Kaohsiung Journal of Medical Sciences》 12卷7期 (1996/07) Pp. 394-399

https://doi.org/10.6452/KJMS.199607.0394

摘要

23位(10位男性，13位女性)促乳素腺瘤的病人藉由經蝶骨下腦垂腺瘤切除手術取得7例微小型和16例巨大型促乳素腺瘤檢體做細胞培養來觀察在生體外激素分泌的情況。培養基中的促乳素濃度在度培養期間可以定量測出，結果顯示培養液中高促乳素的濃度可做為腫瘤早期再發的參考。體外的微小型和巨大型促乳素腺瘤的細胞培養其形態學上的特徵和原始腫瘤的細胞特徵非常相似，大多數巨大型促乳素腺瘤含有濃密且粗糙的分泌顆粒。而細小型則含稀疏的分泌顆粒。促乳素腺瘤的細胞培養技術配合形態學的探討可以研究促乳素腺瘤構造與功能間的相互關係，並且能更進一步的認識這一類腺瘤的生物特性。

並列摘要

Cell cultures of 7 micro- and 16 macroprolactinomas obtained from twenty-three patients (10 males and 13 females) by transsphenoidal adenomectomies were found to be capable of secreting hormones in vitro. Prolactin levels in the culture media were measu-red during the culture periods and showed that the high PRL levels in culture media may indicate carly relapse. Morphological features of both micro- and macropro-lactinomas in vitro resembled those of the original tumors. Most macroprolactinomas harbored densely-granulated cells, while most microprolactinomas sparsely-granulated cells. The cell culture techniques combined with morphological studies of prolactinomas allow further investigation of structure-function correlation in human prolactinomas.

並列關鍵字

prolactinoma ； cel culture