Expression of Epidermal Growth Factor Receptors and C-Erbb-2 Proteins in Human Astrocytic Tumors

上皮生長激素受器和C-erbB-2蛋白在人類星狀細胞瘤的表現

黃旭霖(Shiuh-Lin Hwang) ； 蔡志仁(Chee-Yin Chai) ； 林相如(Hsiang-Ju Lin) ； 洪純隆(Shen-Long Howng)

《The Kaohsiung Journal of Medical Sciences》 13卷7期 (1997/07) Pp. 417-424

https://doi.org/10.6452/KJMS.199707.0417

摘要

基因結構或功能的改變和腫瘤的生成有非常密切的關連。人類腦腫瘤中，各種致癌基因的研究報告相當多，尤其是上皮生長激素受器更是詳盡，然而 c-erbB-2 蛋白在星狀細胞瘤中的研究則顯得相對稀少。由於上皮生長激素受器和 c-erbB-2 蛋白結構類似，但免疫性不同，因此，本研究利用免疫組織染色方法，分析 33 例人類星狀狀細胞瘤中上皮生長激素受器和 c-erbB-2 蛋白的表現。根據世界衛生組織腦腫瘤分類，本研究包括 9 例低惡性度星狀細胞瘤， 15 例分化不良星狀細胞瘤和 9 例多形性神經膠母細胞瘤。上皮生長激素受器的表現可在 28 例腫瘤發現，佔 85 %，其中，低惡性度星狀細胞瘤 6 例（ 67 % ) ，分化不良星狀細胞瘤有 13 例 ( 87 % ) ，而最惡性的神經膠母細胞瘤有 9 例 ( 100 % )。 c- erbB-2 蛋白可在17例星狀細胞瘤發現，佔 51.5 % ，包括 3 例低惡性度星狀細胞瘤（37.5 % ) , 9 例分化不良星狀組飽瘤（81.5 % ) , 和 5 例神經膠母細胞瘤（62.5 %）。研究結果顯示，上皮生長激素受器的表現，隨著星狀細胞瘤惡性度增加而增強，。c-erbB-2蛋白的表現，雖無此趨向，但在較惡性的分化不良星狀細胞瘤和神經膠母細胞瘤中，有較高比例的表現。

並列摘要

Tumorigenesis is the result of sequential or multiple genetic alterations. The• over- expression or amplification of various oncogenes in. diverse human brain tumors have been observed. While numerous studies on the immunohistochemical demonstration of EGFR-overexpression have been reported, little has been found in the literature about the c-erbB-2 protein in human astrocytic tumors. In the present study, we evaluated the expression of EGFR and c-erbB-2 protein in 33 astrocytic tumors with immunohistochemistry. According to the World Health Organization brain tumor classification, the study included 9 low-grade astrocytomas (grade 2), 15 anaplastic astrocytomas (grade 3), and 9 glioblastomas multiforme (grade 4). The positive EGFR immunoreactivity was detected in 28 (85%) of 33 tumors. The expression of EGFR increased with the grade of malignancy in low-grade astrocytomas (67%), anaplastic astrocytomas (87%), and glioblastomas (100%). For the expression of c-erbB-2 protein, 17 (51.5%) of 33 tumors were positive immunostain, including 3 low-grade astrocytomas (37.5%), 9 anaplastic astrocytomas (81.8%), and 5 glioblastomas (62.5%). Different degrees of immunoreactivity for c-erbB-2 protein were found in variant grades of astrocytomas. However, the positive immunostain of EGFR displayed moderate or strong reactivity. The coexpression of EGFR and c-erbB-2 protein was found in 17 (15.5%) of 33 tumors.
The results emphasize that the overexpression of EGFR parallels astrocytoma progession and higher frequency of c-erbB-2 immunoreactivity was seen in snaplastic astrocytomas and glioblastomas than in low-grade astrocytomas.

並列關鍵字

epidermal growth factor receptor ； c-erbB-2 protein