Changes in Lysyl Oxidase Activity and Calcium Content During the Healing of Tooth Extraction Wounds

拔牙創傷癒合過程中離胺基酸氧化酶活性變化與鈣化含量變化之研究

陳靜容(Ching-Jung Chen) ； 康莊(Jun-Kang) ； 謝天渝(Tien-Yu Shieh)

《The Kaohsiung Journal of Medical Sciences》 13卷7期 (1997/07) Pp. 432-439

https://doi.org/10.6452/KJMS.199707.0432

摘要

傷口癒合過程中，不論是結締組織或新骨的形成，膠原蛋白都扮演著極重要的角色。交叉聚合使膠原蛋白成熟，同時增加傷口癒合組織的強度。離肢基酸氧化酣（lysyl oxidase）是目前所知和膠原蛋白交叉聚合有關的唯一酵素，然而它在拔牙傷口癒合時的活性變化情形，到目前都還沒有研究發表過。本實驗係以兔子拔牙傷口的癒合組織為對象，於拔牙手術後 1, 2, 3, 5, 7, 10, 14, 21, 28, 56天分別取出拔牙齒槽內癒合組織，檢測鈣含量，萃取離膠基酸氧化醉並作活性反應，進而分析不同癒合時期之酵素活性變化及其與鈣含量變化間之關係。另取下顎骨以進行放射照像記錄及顯微組織觀察。結果發現，離肢基酸氧化醃之活性於術後第 1 天就能測出，到了第5天後開始急遽增加，至第7天達到最高值。而後酵素活性逐漸降低，至第14天以後回復正常值範圍。在顯微組織觀察下，第7天時，拔牙齒槽內充滿了新生的纖維結締組織，且有類骨質出現”隨著拔牙齒槽內有骨質生成，鈣化發生，鈣含量於術後第7天開始急速增加至第14天，而後維持一平穩含量。以上結果顯示於拔牙傷口癒合初期，大量膠原蛋白生合成時，離肢基酸氧化醃具旺盛的活性表現以促進交叉聚合的發生”當礦物鹽逐漸沈澱，離肢基酸氧化釅活性也明顯降低。

並列摘要

The typical wound healing process after tooth extraction includes several important biological reactions. The collagen fiber, which is the main organic component of connective tissue, plays an important role in the formation of granulation tissue and also bone tissue. Maturation of collagen is controlled by the intermolecular cross- links in the collagen fibers. Lysyl oxidase, which catalyzes the corresponding aIde- hyde formation from certain lysyl and hydroxylysyl residues in collagen and elastln In the first step of the linking reaction, Is the only known enzyme required In the cross- linking process. This study was designed to analyze changes in the lysyl oxidase activity and calcium content during the healing of rabbit tooth extraction wounds and to survey the relationship between them. Healing tissue in the extraction sockets was removed 1, 2, 3, 5, 7, 10, 14, 21, 28, and 56 days postoperatively. After decalcification, lysyl oxidase was extracted from wound tissue, and activity of this enzyme was examined by tritium-release assay. Radiographs and histologic sections were also obtained In each group. The results show that lysyl oxiclase activity can be detected on the first day postoperatively and that it Increases sharply from days five to seven, reaching the peak value on the seventh day. After that, enzyme activity decreases gradually and returns to normal range on day 14. Histologically, the healing sockets were filled mainly with young fibrous connective tissue at day seven; osteold tissue could be found on the lateral wall and fundus of the extraction sockets at the same time. Accompanying the formation of new bone, calcium content Increased conspicuously from day 7 to day 14. These results indicate that lysyl oxidase predominantly acts to promote cross-linking of the abundantly synthesized collagen In the early stage of healing of rabbit tooth extraction wounds. When minerals were precipitated at a great rate in the newly-formed bone, lysyl oxidase activity was concurrently decreased.

並列關鍵字

lysyl oxidase activity ； tooth extraction wound ； calcium content