Effect of Liposomes on Mineralization in Rat Osteoblast-Enriched Cultures

微脂粒在大白鼠造骨母細胞培養中對骨質礦物質化之影響

黃景勝(Jiing-Sheng Huang) ； 吳樺芬(Hue-Fen Wu) ； 洪堅銘(James-Mint Hong) ； 張富雄(Fu-Hsiung Chang) ； 洪基隆(Keelung Hong)

《The Kaohsiung Journal of Medical Sciences》 15卷4期 (1999/04) Pp. 187-194

https://doi.org/10.6452/KJMS.199904.0187

摘要

Liposomes,artificial membranous lipid vesicles, have been used as model structures for biological calcification processes. However, there is no difinite conclusion that liposomes can bo like matrix vesicles for inducing bone calcification and bonelike tissue formation on primary cultured cells. To determine whether liposomes can promote bone cell growth and mineraliation by inducing crystal nucleation, liposomes composed of egg phosphatidylcholine, cholesterol, and bovine brain phosphatidylserine were added to 21-day-old Sprague-Dawley fetal rat calvarial cell cultures from day 1. The aims were to observe proliferation and the phenotype of osteoblasts by measuring cell numbers and alkaline phosphatase(ALP) activity and, when added at confluence, to observe calcification. The date were analyzed with two-way ANOVA. During the 16-day culture period, cell numbers were not significantly affected by liposomes (100µ mol/L). However, ALP activeity was significantly inhibited by liposomes (P＜0.05) at day 16 and thereafter. Calcified particles were detected by von kossa’s method, and were larger and more abundant (p＜0.05) in the liposomes groups than in the control from days 12-24. This reponse depended on liposomes dose. These findings suggest that liposomes promote calcification and accelerate the formation of bone-like tissue. Liposomes slightly reduce the expression of the osteoblast phenotype and do not affect cell growth in primary rat osteoblast-enriched cultures.

並列摘要

Liposomes,artificial membranous lipid vesicles, have been used as model structures for biological calcification processes. However, there is no difinite conclusion that liposomes can bo like matrix vesicles for inducing bone calcification and bonelike tissue formation on primary cultured cells. To determine whether liposomes can promote bone cell growth and mineraliation by inducing crystal nucleation, liposomes composed of egg phosphatidylcholine, cholesterol, and bovine brain phosphatidylserine were added to 21-day-old Sprague-Dawley fetal rat calvarial cell cultures from day 1. The aims were to observe proliferation and the phenotype of osteoblasts by measuring cell numbers and alkaline phosphatase(ALP) activity and, when added at confluence, to observe calcification. The date were analyzed with two-way ANOVA. During the 16-day culture period, cell numbers were not significantly affected by liposomes (100µ mol/L). However, ALP activeity was significantly inhibited by liposomes (P＜0.05) at day 16 and thereafter. Calcified particles were detected by von kossa’s method, and were larger and more abundant (p＜0.05) in the liposomes groups than in the control from days 12-24. This reponse depended on liposomes dose. These findings suggest that liposomes promote calcification and accelerate the formation of bone-like tissue. Liposomes slightly reduce the expression of the osteoblast phenotype and do not affect cell growth in primary rat osteoblast-enriched cultures.

並列關鍵字

liposomes ； mineralzation ； osteoblast