Comparison of Necrotic Characteristics and Benefits between 50% Acetic Acid and Pure Ethanol in Local Hepatic Injection: a Study in Rats

醋酸及純酒精於肝臟局部注射之壞死能力及特性的比較 －動物實驗

侯丞(Cheng Hou) ； 陳信成(Shinn-Cherng CDhen) ； 張文宇(Wen-Yu Chang) ； 陳謙信(Chien-Hsin Chen)

《The Kaohsiung Journal of Medical Sciences》 15卷7期 (1999/07) Pp. 414-418

https://doi.org/10.6452/KJMS.199907.0414

摘要

局部注射治療於小型肝癌之治療效果目前已被廣泛接受，但尋找更佳的藥物以減少局部殘餘的癌細胞，進而減少局部復發的機會，一直是大家努力的目標之一。本實驗之目的在於比較醋酸及純酒精於肝臟局部注射時其破壞能力之差異。本研究以12隻Sprague－Dawley Rats為對象，重量約260－300公克。Pentobarbital腹腔注射麻醉後，打開腹腔，將0.03ml之純酒精及0.01ml之50％醋酸分別注射於同一隻老鼠之肝臟左葉表面的不同部位，分別觀於第0,5,及15分鐘時，其壞死之形狀及破壞範圍的徑於第0,5,及15分鐘時均為1.5±0.58 mm並無明顯之變化。但50％醋酸這組其壞死直徑於第0,5,及15分鐘將分別為2.62 ± 0.58，4.50 ± 0.84，及5.25 ± 0.75 mm 呈現明顯之浸潤現象。（0'及5'P＜0.0001，將5'及15'P＜0.001）而0至15分鐘之平均擴散半徑為1.33mm。純酒精於肝臟局部注射後無明顯擴散現象，且形狀為不規則形。50％醋酸於局部注射後，則呈現明顯之擴散現象，且形狀擴散為圓形或橢圓形。因此醋酸應用於局部注射治療上，其效果可能更優於純酒精。

並列摘要

This study compares the characteristics of acetic acid and pure ethanol in local hepatic injection and identifies the possible benefits from acetic acid by an animal model. We injected 0.03 ml of pure ethanol and 0.01 ml of 50% acetic acid separately into different sites of the same liver. The shape and diameter of the lesions were recorded at 0 min, 5 min and 15 min, and 5 lesions were also reviewed one day later. The lesions induced by pure ethanol were irregular, whereas those induced by 50% acetic acid were round or oval. The diameter of lesions induced by 50% acetic acid was 2.62 ± 0.58 mm initially and increased to 4.50 ± 0.84 mm and 5.25 ± 0.75 mm at 5min and 15 min, (5min compared with 0 min, p＜0.0001; 15 min compared with 5 min, p＜0.001) respectively. The diameters of lesions induced by pure ethanol did not increase. The diffusion diameter at 5 min and 15 min were 1.88 mm and 2.63 mm respectively. Furthermore, viable liver cells were occasionally found in two lesions induced by pure ethanol. Acetic acid had a more homogeneous distribution and better infiltrating ability than pure ethanol and may be superior to pure ethanol in local injection therapy of HCC. But more clinical trials in the treatment HCC should be carried out.

並列關鍵字

acetic acid ； hepatic injection ； pure ethanol