Seroprevalence of Cmv Antibodies in a Blood Donor Population and Premature Neonats in The South-Central Taiwan

中、南台灣捐血者及早產兒巨細胞病毒抗體盛行率之研究

盧世乾(Shih-Chien Lu) ； 金立德(Li-Te Chin) ； 吳福明(Fu-Ming Wu) ； 謝國珍(Gow-Jen Hsieh) ； 黃上邦(Shang-Pang Haung) ； 陳榮洲(Jung-Chou Chen) ； 張貞祥(Andrew Cheng-Hsiang Chang) ； 謝文貴(Wen-Kuei Hsieh) ； 陳百薰(Bai-Hsiun Chen)

《The Kaohsiung Journal of Medical Sciences》 15卷10期 (1999/10) Pp. 603-610

https://doi.org/10.6452/KJMS.199910.0603

摘要

經由輸血感染巨細胞病毒可能會對免疫力弱的病人造成危險。為了評估提供無巨細胞病毒之血液的可能性，吾人自台南捐血中心連續收集1 , 800個捐血者血清檢體，以酵素免疫分析法(EIA)來測定巨細胞病毒抗體的盛行率，結果發現其中只有150個檢體(8.3 %)呈陰性反應。此抗體陰性率在男女間無有意義之差異。巨細胞病毒抗體陰性率隨年齡群增加而減少。為了更進一步瞭解巨細胞病毒抗體和其它血液篩檢項目之相關性，吾人在同批檢體中比較巨細胞病毒抗體陽性者和陰性者其 B 型肝炎表面抗原、C型肝炎抗禮、第一型暨第二型人類免疫缺乏病毒（ HIV ）抗體和第一型暨第二型人類嗜 T 淋巴球病毒(HTLV)抗體之陽性率，結果發現這些陽性率在兩者間無有意義之差異。吾人用聚合酵素增生反應進行核酸增殖亦發現在本地區的二十個早產兒檢體中有六個(33.3 %)得到陽性結果，顯示先天性巨細胞病毒感染之比率亦相當顯著。綜合以上結果得知：因為本地是巨細胞病毒感染高流行區，故在本地區提供巨細胞病毒抗體陰性的血液給需要輸血者不能僅靠巨細胞病毒抗體篩檢，其他方法如聚合酵素增生反應或使用過濾器去除白血球可能更為可行。

並列摘要

nfection of cytomegalovirus (CMV) via contaminated blood may endanger immunocompromised patients that require transfusion therapy. The aim of this study is to determine the prevalence of CMV antibodies in the blood donor population in Southern-central Taiwan. A total of 1800 consecutive sera, obtained from Tainan Blood Center of Chinese Blood Services Foundation (CBSF), were tested for CMV antibodies by two commercial enzyme immunoassays (EIAs). Of the sera tested, 150 (8.3%) were found to be CMV seronegative. The frequency of CMV seropositivity revealed no significant difference between male and female donors. The frequency of CMV seronegativity showed a stepwise decrease with the increase of donor age. In addition, the prevalence of HBsAg, antibodies to hepatitis C virus (anti-HCV), antibodies to human immunodeficiency viruses type 1 and 2 (anti-HIV 1+2) and antibodies to human T-cell lymphotropic viruses type I and II (anti-HTLV 1/11) were compared between CMV seropositive and seronegative groups. Our results showed that there was no significant difference in seroprevalence of these markers between CMV seropositive and seronegative groups. Our findings also showed that six out of twenty (30.0%) premature neonates were CMV-seropositive. These premature specimens and those EIA discrepancy samples were confirmed by specific nucleic acid amplification using polymerase chain reaction (PCR). Our results suggest that a program which aims to supply CMV seronegative blood or blood components to the patients, should not solely depend on current antibody screening methods in an area where CMV infection is highly endemic. Amendments such as PCR testing, leukocyte reduction by filtration before transfusion may be more pract

並列關鍵字

cytomegalovirus (Cmv) ； EIA ； CMV antibodies