Effects of Drugs on Cellular Proliferation in Cultured Iris Pigment Epithelial Cells and Retinal Pigment Epithelial Cells

藥物對虹膜色素上皮細胞及視網膜上皮細胞增生的影響

吳國揚(Kwou-Yeung Wu) ； 洪秀貞(Show-Jen Hong) ； 王惠珠(Hwei-Zu Wang) ； 黃佳惠(Jia-Huae Hwang) ； 賴昱宏(Yu-Hung Lai)

《The Kaohsiung Journal of Medical Sciences》 17卷2期 (2001/02) Pp. 77-83

https://doi.org/10.6452/KJMS.200102.0077

摘要

本實驗主要是探討不同種類的藥包括methionine-enkephlin, isoproterenol, dibutyryl cAMP, endothelin-1, dexamethasone及phorbol 12-myristate 13-acetate(PMA)對體外培養的豬眼球虹膜色素上皮細胞及視網膜色素上皮細胞對3H-thymidine攝取量的影響，結果顯示兩種細胞經過體外培養後皆呈現表皮狀的形態，兩種細胞內亦含有許多色素顆粒。虹膜色素上皮細胞經過endothelin-1處理後，細胞對3H-thymidine攝取量增加到控制組的126%，但是isoproterenol卻降低虹膜色素上皮細胞的攝取量只剩下控制組的83%，其它的藥例如methionine-enkephalin, dibutyryl camp, dexamethasone及PMA都不影響虹膜色素上皮細胞的增生。然而藥物對視網膜色素上皮細胞的增生卻有不同的影響，結果顯示methionine-enkephalin及isoproterenol皆分別可增加攝取量到控制組的145%及146%，但是dibutyryl camp，dexamethasone及PMA卻會抑制視網膜色素上皮細胞對3H-thymidine攝取量分別只剩下83%，73%及85%，而endothelin-1對視網膜色素上皮細胞的增生卻沒有影響，由以上結果顯示雖然虹膜色素上皮細胞及視網膜色素上皮細胞二者的形態類似，但是調節二種色素細胞的增生機轉可能不相同。

並列摘要

In this study, iris and retinal pigment epithelial cells were cultured from porcine and various drugs including methionine-enkephalin, isoproterenol, dibutyryl cAMP, endothelin-l, dexamethasone and phorbol12-myristate 13-acetate (PMA) were used to investigate their effects on both cellular proliferation in cultured porcine iris and retinal pigment epithelial cells. Cellular proliferation was estimated with 3H-thymidine uptake. It is indicated that both pigment epithelial cells possess epithelial-like morphology and abundant pigment granules in cells obviously. Following the iris pigment epithelial cells being treated with endothelinl, the 3H-thymidine uptake in the cells was increased to 126% as compared with the control. However, the cellular proliferation was decreased to 830/0 when the cells were treated with isoproterenol. In the case of methionine-enkephalin, dibutyryl cAMP, dexamethasone and phorbol 12-myristate 13-acetate (PMA), he thymidine uptake in the iris pigment epithelial cells was not affected by above drugs. In the retinal pigment epithelial cells, the 3H-thymidine uptakes were increased to 145% and 146% when the cells were incubated with methionine enkephalin, and isoproterenol, respectively. In the presence of dibutyryl cAMP, dexamethasone and phorbol ester (PMA), the cellular proliferation was-inhibited to 83%, 73% and 85% respectively. However, endothelin-l did not affect the cellular proliferation in retinal pigment epithelial cells. These results show that the morphological shapes of iris pigment epithelial cells are similar to retinal pigment epithelial cells. However, the cellular proliferation in both cells may be regulated by distinct mechanisms.

並列關鍵字

iris pigment epithelial cells ； retinal pigment epithelial cells ； cell proliferation