Mucin Gene Expression in Gallbladder Epithelium with Black Pigment Stone Ascertained by in Situ Hybridization

含黑色膽石之膽囊上皮細胞之粘液基因排列

李金德(King-Teh Lee) ； 劉燦鑫(Tsan-Shium Liu)

《The Kaohsiung Journal of Medical Sciences》 17卷10期 (2001/10) Pp. 517-523

https://doi.org/10.6452/KJMS.200110.0517

摘要

黑色膽石成因異於膽色素結石或膽固醇結石，它主要形成於肝硬化或溶血性病人的膽囊內。一般認為膽石症的形成與膽汁內的粘液醣蛋白有關，而粘液醣蛋白主要來自膽上皮細胞，因此本研究亟想探討含黑色膽石的膽囊上皮細胞，其膽囊上皮細胞之粘液糖蛋白的基因排列與正常不含結石的膽囊上皮細胞有何差異。我們以含黑色結石之膽囊上皮10例為研究對象，並以6位正常膽囊為對照組，以in situ hybridization的方法對MUC1-4,MUC5B及MUC6的探測，結果我們發現（1）所有的黑色結石的膽囊上皮粘液素皆含有MUC1,MUC3,MUC5B及MUC6，而正常膽囊相對的僅有33.3%,83.3%及66.7%的膽囊上皮含有該類基因排列，亦即黑色結石的膽囊上皮粘液的表現增加(2)MUC2及MUC4未在正常膽囊上皮出現，卻出現於20%及60%的黑色結石膽囊上皮，因此我們認為含黑色結石的膽囊上皮細胞，其粘液基因的表現確實異於正常的膽囊上皮細胞；而黑色結石膽囊上皮的粘液基因的表現，以MUC2,MUC5B及MUC6最明顯，這些粘液素對結石的形成有相當大的關係。

並列摘要

Black pigment stones are usually found in patients with liver cirrhosis or hemolytic disease. Mucoglycoproteins are present in a significant amount in black pigment stones and contribute to the matrix of gallstones. Epithelium of stone-containing gallbladders contains much more mucin than those without stones. In this study, we try to determine by in situ hybridization the mucin gene expression in black stone-containing gallbladders and try to find the diversity of mucin gene expression in gallbladders containing black pigment stones and those without stones. In situ hybridization with DIG-tailed oligonucle-otides was performed on sections of paraffin-embedded tissues of gallbladders with black pigment stones (n=10) and those without stones (n=6) to identify the expression of MUC1, MUC2, MUC3, MUC4, MUC5B and MUC6 in gallbladder epithelium. The findings showed that (1) mRNA expression of MUC1, MUC3, MUC5B and MUC6 were found in all gallbladders with black pigment stones, while they were expressed in 33.3%, 83.3%, 83.3% and 66.7% respectively in those without stones. They were expressed more strongly and extensively in gallbladders with stones when compared to those without stones. (2) MUC2 and MUC4 labeling were absent in gallbladders without stones, while they were present in 20% and 60% of gallbladders with black pigment stones, respectively. We conclude that MUC3, MUC5B and MUC6 were the main mucin gene expression in either gallbladder with or without stones. Altered mucin gene expression occurred in gallbladders with black pigment stones, such as the presence of MUC2 and MUC4 and increased expression of MUC1, MUC3, MUC5B and MUC6 in black stone-containing gallbladders. The higher incidence and stronger labeling intensity of mucin gene expression of MUC2, MUC3, MUC5B and MUC6 in black stone-containing gallbladder may reflect abundant mucin content in these gallbladders. Increased expression of MUC2 and MUC4 in black stone-containing gallbladder epithelium indicated that intestinal metaplasia and altered mucin genes could occur in diseased gallbladders.

並列關鍵字

mucin ； gene expression ； gallbladder ； black pigment stone