Effects of Crude Drugs on Lipolysis in Differentiated 3T3-L１ Adipocytes

中藥對3T3-L1脂肪細胞脂肪分解的作用

洪秀貞(Show-Jen Hong) ； 馮濟敏(Jim. C Fong) ； 黃佳惠(Jia-Huae Hwang)

《The Kaohsiung Journal of Medical Sciences》 18卷4期 (2002/04) Pp. 157-163

https://doi.org/10.6452/KJMS.200204.0157

摘要

本實驗主要是探討六種用水抽出的中藥成份對3T3-L1脂肪細胞之脂肪分解作用的影響；六種中藥包括人參、生地黃、葛根、黃耆、地骨皮及黃芩，而脂肪分解作用是以測量細胞釋出的甘油量當做指標。結果發現3T3-L1脂肪細胞經過不同濃度藥物處理60分鐘後，3T3-L1脂肪細胞釋出甘油的量在控制組是71nmole/mg protein，經人參處理後細胞釋出甘油的量降為48(0.1mg/ml)，46(1mg/ml)及31nmole/mg protein (10mg/ml)，生地黃影響甘油釋出量則降為60(0.1mg/ml)26(1mg/ml)及20 nmole/mg protein (10mg/ml)葛根對甘油釋出量降為35(0.1mg/ml)，34(1mg/ml)及30nmole/mg protein(10mg/ml)，黃耆對甘油釋出量降為108(0.1mg/ml)，73(1mg/ml)及70nmole/mg protein(10mg/ml)，地骨皮對甘油釋出量降為126(0.1mg/ml)，112(1mg/ml)及nmole/mg protein90(10 mg/ml)，黃芩對甘油釋出量降為118(0.1 mg/ml)，77(1mg/ml)及29nmole/mg protein(10mg/ml)。細胞經過10-6M isoproterenol處理後，控制組的甘油釋出量則增加到169nmole/mg protein，經人參處理後，甘油釋出量降為76(0.1mg/ml)，73(1mg/ml)，及72nmole/mg protein(10mg/ml)，生地黃對甘油釋出量降為52(0.1mg/ml)，35(1mg/ml)及11nmole/mg protein(10mg/ml)，葛根對甘油釋出量降為26(0.1mg/ml)，25(1mg/ml)及20nmole/mg protein(10mg/ml)，黃耆對甘油出量降為160(0.1mg/ml)，92(1mg/ml)及88nmole mg protein(10mg/ml)，黃芩對甘油釋出量則降為149(0.1mg/ml)，地骨皮對甘油釋出量降為160(0.1mg/ml)，92(1mg/ml)及88nmole/mg protein(10mg/ml)。由以上果顯示不論在缺乏或有isoproterenol存在下，適當濃度的人參、生地黃及葛根等中藥之水溶性的抽出物都會降低3T3-L1脂肪細胞對脂肪分解的作用。

並列摘要

In the present study, aqueous fractions extracted from Radix Ginseng, Radix Rehmanniae, Radix Puerariae, Radix Asparagi, Cortex Phellodendri and Radix Scutellariae were investigated for their effects on lipolysis measured the glycerol release in cultured 3T3-Ll differentiated adipocytes cells. Following treatment of cells with various concentrations of water-soluble extracts ranging from 0.1, 1 to 10 mg/ml for 60 mim, the basal glycerol release from 3T3-Ll cells was changed from 71 nmole/mg protein of control to 48, 46 and 31 nmole/mg protein in Radix Ginseng-treated cells. Amount of glycerol was reduced to 60, 26 and 20 by Radix Rel1manniae. In the presence of Radix Puerariae, glycerol release was decreased to 35, 34 and 30, respectively. After exposure to Radix Asparagi, amount of glycerol became 108, 73 and 70 nmole/mg protein, respectively. In the case of Cortex Phellodendri, amount of glycerol was increased from 126, 112 to 90, respectively. In the presence of Radix Scutellariae, the glycerol was changed to 118, 77 and 29, respectively. In isoproterenol-stimulated cells, the glycerol release from cells by Radix Ginseng was changed from 169 of control to 76, 73 and 72 nmole/mg protein, respectively. After incubation with Radix Rehmanniae, amount of glycerol decreased to 52,35 and 11, respectively. In the presence of Radix Puerariae, the glycerol was changed to 26, 25 to 20, respectively. In the presence of Radix Asparagi, the glycerol became 160,96 and 64, respectively. In the case of Cortex Phellodendri, the glycerol was increased to 160, 92 to 88, respectively. In the presence of Radix Scutellariae, the glycerol was changed to 149,83 and 50, respectively. These results indicated that the water-soluble substances from Radix Ginseng, Radix Rehmanniae and Radix Puerariae decreased the lipolysis in basal and isoproterenol-stimulated 3T3-Ll adipocytes.

並列關鍵字

lipolysis ； glycerol release ； 3T3-Ll adipocytes ； crude drugs