Expression of Hsp72 in Lymphocytes in Patients with Febrile Convulsion

熱休克蛋白72在熱痙攣病人淋巴球的表現

林龍昌(Lung-Chang Lin) ； 陳香文(Hsiang-Wen Chen) ； 楊瑞成(Rei-Cheng Yang)

《The Kaohsiung Journal of Medical Sciences》 21卷3期 (2005/03) Pp. 101-107

摘要

雖然熱痙攣在小兒神經科的領域中是一種相當常見的疾病，但目前爲止對它的致病機轉還不是很清楚。在這個研究中，我們主要探討熱休克蛋白在熱痙攣所扮演的角色。十個年齡從九個月到三歲八個月的熱痙攣病人，八男二女，當做實驗組；另外找十個年齡相當的正常兒童作爲對照組，分別抽取他們的淋巴球做熱休克蛋白72合成動力學的分析，結果顯示兩組在熱休克處理前都可以測到微量的熱休克蛋白72，在熱休克處理後，兩組的熱休克蛋白72都有明顯的增加，但兩組增加的量並沒有顯著的差異。但是在蛋白質磷酸化的探討上，熱痙攣病人這一組熱休克蛋白72的磷酸化卻明顯比對照組少。我們認爲，熱休克蛋白的磷酸化也許是熱痙攣的致病機轉之一。

關鍵字

熱痙攣 ； 熱休克蛋白 ； 磷酸化

並列摘要

The pathophysiology of febrile convulsion, the most common childhood neurologic disease, remains unclear. In this study, we investigated what role a heat shock protein plays in this disease. We enrolled eight boys and two girls with febrile convulsion and 10 age-matched healthy controls. We did a biosynthetic evaluation of both groups by separating lymphocytes and measuring the expression of heat shock protein 72 before and after heat shock treatment. Before the treatment, both groups were found to have small amounts of constitutive heat shock protein 72. Afterwards, its expression increased in both groups, and no statistical difference was found between the increases in the two groups. In addition, there was no obvious difference in the susceptibility to produce heat shock proteins. However, the febrile convulsion group was found to have a significant decrease in phosphorylation of heat shock protein 72. These results suggest the possible involvement of post-translational modification of heat shock proteins, most likely phosphorylation, in the pathogenesis of febrile convulsion.

並列關鍵字

febrile convulsion ； heat shock protein ； phosphorylation