Effects of Antibiotics and Corticosteroid Eyedrops on Cellular Proliferation in Cultured Human Corneal Keratocytes

抗生素及類固醇眼藥水對人類角膜纖維母細胞增生之影響

吳國揚(Kwou-Yeung Wu) ； 王惠珠(Hwei-Zu Wang) ； 洪秀貞(Show-Jen Hong)

《The Kaohsiung Journal of Medical Sciences》 22卷8期 (2006/08) Pp. 385-389

摘要

本實驗之主要目的是研究不同的抗生素包括gentamicin，sulfisomezole及類固醇fluorometholone，dexamethasone及betamethasone眼藥水對人類角膜纖維母細胞增生的影響。人類角膜纖維母細胞經RPMI-1640及10%胎牛血清培養後加入經1/10，1/100及1/1,000稀釋的上述眼藥水，經過100分鐘培養後測試細胞對[3H]-thymidine的攝取量。結果發現三種稀釋濃度的gentamicin都不影響細胞的增生，但是細胞對[3H]-thymidine的攝取量會被1/10、1/100稀釋的sulfisomezole抑制到只有控制組的82%及90%，也被1/10稀釋的fluorometholone及1/10，1/100稀釋的betamethasone抑制到只有控制組的75%、84%及86%，而且1/10、1/100及1/1,000稀釋的dexamethasone皆會明顯抑制細胞增生速率到只有控制組的82%，86%及90%。因此本實驗顯示類固醇眼藥水會抑制人類角膜纖維母細胞的增生而可能會改變角膜基質組織傷口愈合的過程。

關鍵字

betamethasone ； 細胞增生 ； 角膜纖維母細胞 ； dexamethasone ； fluorometholone ； sulfisomezole

並列摘要

The purpose of this study is to investigate the effects of antibiotics and glucocorticoid eyedrops, including gentamicin, sulfisomezole, fluorometholone, dexamethasone, and betamethasone, on cellular proliferation in cultured human corneal keratocytes. Human corneal keratocytes were cultured in RPMI-1640 containing 10% fetal bovine serum. Drugs were prepared from original concentrations to 1/10, 1/100, and 1/1,000 dilutions. After exposure to drugs for 100 minutes, cellular proliferation was estimated by [3H]-thymidine uptake. It was found that cellular proliferation in corneal keratocytes was not affected by any of the three dilutions of gentamicin but was inhibited by 1/10 and 1/100 dilutions of sulfisomezole to 82% and 90% of control. [3H]-thymidine uptake values were inhibited to 75% by 1/10 dilution of fluorometholone and by 1/10 and 1/100 dilutions of betamethasone to 84% and 86% of control. Meanwhile, cellular proliferation was significantly inhibited by 1/10, 1/100, and 1/1,000 dilutions of dexamethasone to 82%, 86%, and 90%, respectively, in comparison with control values. It was demonstrated that commercial eyedrops of glucocorticoids inhibit cellular proliferation in corneal keratocytes, which may modulate the wound healing of corneal stroma.

並列關鍵字

betamethasone ； cellular proliferation ； corneal keratocytes ； dexamethasone ； fluorometholone ； sulfisomezole