- 期刊
Effect of Detergents on Mitochondria Isolated from RAT Myocardium
洗滌劑對大白鼠心肌粒腺體的影響
摘要
從大白鼠心臟分離出來的粒腺體在形態上很完整，代謝上亦十分活耀，但是將它們混合在mannitol buffer中成懸浮液狀再經30分鐘37℃溫浴，其形態就有明顯的腫脹而其質膜也失去整體性，同時測量出的遊離脂肪酸(Free Fatty Acids)也較沒有溫浴時多出2到3倍，在溫浴時加入溶磷脂(Lysophosphatidylcholine)則更使質膜破損形成更多胞體但並不釋放更多的遊離脂肪酸。加入洗濯劑(Detergents)無論Deoxycholate(1 mg/ml)或Triton X-100(0.01%)則會抑制粒腺體釋放更多遊離脂肪酸，但仍嚴重的破壞質膜形態。因此可說粒腺體腫脹及質膜失去整體性而導致的結果與由洗濯劑所導致的磷脂解體釋放出遊離脂肪酸相附和，而結果也指出在缺血缺氧時由大白鼠心臟分離出的粒腺體是很容易發生腫脹的形態變化而質膜上的磷脂也因此而被分解產生二種自然的洗濯劑，遊離脂肪酸及溶磷脂。
關鍵字
無資料並列摘要
Sun, S. H., C. J. Lin, H. H. Sun, G. Y. Sun and B. N. Chiang: Effect of detergents on mitochondria isolated from rat myocardium, Chinese J.Physiol.27(1):63-74,1984.Mitochondria isolated from rat myocardium were morphologically intact and metabolically active. However, upon incubation of the mitochondria in mannitol buffer at 37℃for 30min, there was obvious swelling and loss of structural integrity. The morphological change is correlated with a 2 to 3-fold increase in free fatty acids(FFA)Addition of lysophosphatidylcholine (LPC)to the incubation medium elicited no further increasein the FFA level as compared to samples which were incubated for 30min, but LPC resulted in further disruption of the mitochondrial membrane as shown by an increased amount of vesicles formed from the detached cristae. Detergents like deoxycholate (1 mg/ml)and Triton X-100(0.01%)inhibited the mitochondrial FFA release, but did not protect the mitochondria from loss of structural integrity. It is concluded from these results that phospholipid degradation and release of FFA is associated with mitochondria swelling and loss of respiratory activity. While FFA is probably responsible for uncoupling the mitochondrial oxidative phosphorylation, the loss of membrane integrity is probably due to the detergent effects of lysophospholipids. The results are extended to explaining the morphological and biochemical changes of mitochondria in myocardium after an acute ischemic insult.