Attenuation of Natriuretic Response to Saline Loading in AV3V Lesioned Rats

本實驗旨在比較正常大白鼠及第三腦室前腹區破壞鼠於大量灌注鹽水後之腎臟排泄功能的變化，藉以探討第三腦室前腹區對調節鈉及體液平衡的作用。第三腦室前腹區的破壞是以陽極直流電（3毫安培）通電20秒達成。對照鼠則經相同手術但不通電流。經四週後，兩組大白鼠分別在麻醉或不麻醉（清醒）狀況下進行實驗。在麻醉下，動物由頸靜脈以每分鐘0.388毫升的速率灌注0.9%的生理鹽水，持續一小時，並測定動脈血壓及腎臟的排泄反應之變化。在對照期，兩組大白鼠的動脈壓及腎小球濾過率並無顯著差異；而在大量灌注鹽水後，兩組動物的動脈壓及腎小球濾過率亦無顯著差別。對照鼠因應大量鹽水灌注而鉅幅地增加尿流量及鈉和鉀之排泄率，實驗鼠之腎臟排泄率亦增高，但所增加之幅度僅爲對照鼠者的一半。在清醒狀況下，兩組動物經由口腔灌入量爲體重10%的生理鹽水後，亦見到相似的腎臟排泄反應及反應程度之差異。上述結果顯示第三腦室前腹區與大白鼠水分及鈉的平衡有密切的關係。

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並列摘要

Tsai, L.M., C.C. Lin, W.C. Huang, and T.K. Young: Attenuation of natriuretic response to saline loading in AV3V lesioned rats. Chinese J. Physiol.29(l): 3 1-41, 1986. Anteroventral third ventricle (AV3V) has been implicated in body fluid balance and blood pressure control. This study thus was to evaluate the renal response to saline loading in rats with and without AV3V lesion. Lesioned rats were prepared by electrolytic ablation 4 weeks prior to experiments. Arterial pressure (AP) and renal excretory responses were measured before and after intravenous infusion of 154mM NaCl (0.388ml/min) for 1 hour in anesthetized control and lesioned rats. There were no significant differences in AP (125 ± 3 vs 121 ± 3 mmHg) and glomerular filtration rate (GFR; 1.5 ± 0.1 vs 1.6 ± 0.3 ml/min/g kw) between control rats and lesioned rats. Saline loading did not significantly change AP and GFR in both groups of rats. In control rats there was a striking natriuretic (△2660%) and diuretic (△1870%) response to saline Loading but the lesioned rats only exhibited 50% of the normal excretory response. A similarly attenuated natriuresis and diuresis was also found in conscious, lesioned rats with oral saline loading. These data suggest that AV3V exerts substantial influence on the regulation of sodium and water balance.

並列關鍵字

Anteroventral third ventricle ； saline loading ； glomerular filtration rate ； natriuresis ； diuresis

國際替代計量

Attenuation of Natriuretic Response to Saline Loading in AV3V Lesioned Rats

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