Effect of Estradiol on the Activities of Ornithine Decarboxylase and S-adenosyl-methionine Decarboxylase in Tissues of Ovariectomized Rats

雌二醇對去卵巢老鼠組織內多胺合成脫羧酶活性之影響

陳麗玉(Lih-Yuh C. Wing)

《The Chinese Journal of Physiology》 31卷2期 (1988/12) Pp. 95-103

摘要

多胺和細胞之生長及分化有十分密切的關係。鳥胺酸脫羧酶及腺甘甲硫氨酸脫羧酶爲多胺合成之控制酵素。本文主要是研究雌二醇對子宮、肝臟及腎臟之生長及鳥胺酸脫羧酶，腺甘甲硫氨酸脫羧酶活性之影響。去卵巢之雌老鼠經雌二醇處理後，子宮重量增加，鳥胺酸脫羧酶活性於6小時及18~24小時分别達到高峯期。腎臟之重量不改變，但在18~24小時，鳥胺酸脫羧酶活性有個高峯期。肝臟則不受雌二醇之刺激。連績注射雌二醇，可刺激子宮之生長，鳥胺酸脫羧酶及腺甘甲硫氨酸脫羧酶活性。而對腎臟而言，則只有鳥胺酸脫羧酶活性有顯著堪加，重量則無變化。我們的實驗結果顯示，雖然子宮、腎臟及肝臟均含有雌性素接受器，雌二醇對這三種器官內之多胺合成酵素活性有不同的影響，而這些酵素活性未必和生長狀態有絕對的關聯。

並列摘要

Wing, L.C.: Effect of estradiol on the activities of ornithine decarboxylase and S-adenosyl-methionine decarboxylase in tissues of overiectomized rats. Chinese J. Physiol. 31(2): 95-103, 1988. Polyamines play an important role in cellular growth and differentiation. Ornithine decarboxylase (ODC) and S-adenosyl-methionine decarboxylase (SAMD) catalyze the rate-limiting steps of polyamine formation. Many hormones have been known to affect these decarboxylase activities in their target tissues. This study investigated the effect of estradiol (E2)on the activities of ODC and SAMD and on the growth of three estrogen receptor-containing organs: uterus, kidney and liver. Two weeks after ovariectomy, Sprague-Dawley rats received a subcutaneous injection of E2 (10 μg/rat). The ODC activity and organ weight were determined at various intervals after E2 adminstration. The E2 treatment increased the weight of uterus but not that of liver and kidney. In the uterus, ODC activity showed biphasic increases at 6 h and 16-24 h respectively after E2 injection. Renal ODC activity increased gradually and peaked at 18-24 h whereas hepatic ODC activity remained unchanged. Daily injection of E2 over a 3-day period provoked an increase of uterine weight, uterine ODC and SAMD activity. The same treatment also increased renal ODC activity, however it had no effect on the renal SAMD activity and the kidney weight. Our results indicate that E2 has different effects on the activities of ODC and SAMD in three organs tested, and the increase of ODC activity is not necessarily correlated with the tissue growth.

並列關鍵字

estradiol ； ornithine decarboxylase ； S-adenosyl-methionine decarboxylase ； ovariectomized rats