Weems, C. W., Y. S. Weems, C. N. Lee, and D. L. Vincent. The purine adenosine amplifies the response to gonadotropin and inhibits prostaglandin F(subscript 2α)(PGF(subscript 2α)) suppression of gonadotropin stimulation of bovine luteal cells in vitro. Chinese J. PhysioL 34(4): 355-369, 1991. Dispersed bovine luteal cells collected on day 7 or 13 postestrus were incubated in vitro with human chorionic gonadotropin (hCG) or prostaglandins E1 or E2 (PGE1 PGE2) in the presence or absence of adenosine, dipyridamole and/or prostaglandin F(subscript 2α)(PGF(subscript 2α)). Secretion of progesterone by day 7 or 13 bovine luteal cells was increased by hCG, PGE1 or PGE2 which was increased further when co-incubated with adenosine (P<0.05). Adenosine alone increased secretion of progesterone by day 7 luteal cells (P<0.05) but not by day 13 luteal cells (P>0.05). PGF(subscript 2α) inhibited the gonadotropic response to hCG, PGE1 or PGE2 (P<0.05) which was reduced by adenosine (P<0.05) by day 7 or 13 bovine luteal cell suspensions. Dipyridamole reduced the adenosine-facilitated response to hCG, PGE1 or PGE2 by day 7 or 13 luteal cell suspensions (P<0.05). It is concluded that adenosine enhances the steroidogenic response by functional bovine luteal cells to hCG, PGE1 or PGE2 and that adenosine reduces the luteolytic response to PGF(subscript 2α) by gonadotropin-stimulated bovine luteal cells in vitro.
Weems, C. W., Y. S. Weems, C. N. Lee, and D. L. Vincent. The purine adenosine amplifies the response to gonadotropin and inhibits prostaglandin F(subscript 2α)(PGF(subscript 2α)) suppression of gonadotropin stimulation of bovine luteal cells in vitro. Chinese J. PhysioL 34(4): 355-369, 1991. Dispersed bovine luteal cells collected on day 7 or 13 postestrus were incubated in vitro with human chorionic gonadotropin (hCG) or prostaglandins E1 or E2 (PGE1 PGE2) in the presence or absence of adenosine, dipyridamole and/or prostaglandin F(subscript 2α)(PGF(subscript 2α)). Secretion of progesterone by day 7 or 13 bovine luteal cells was increased by hCG, PGE1 or PGE2 which was increased further when co-incubated with adenosine (P<0.05). Adenosine alone increased secretion of progesterone by day 7 luteal cells (P<0.05) but not by day 13 luteal cells (P>0.05). PGF(subscript 2α) inhibited the gonadotropic response to hCG, PGE1 or PGE2 (P<0.05) which was reduced by adenosine (P<0.05) by day 7 or 13 bovine luteal cell suspensions. Dipyridamole reduced the adenosine-facilitated response to hCG, PGE1 or PGE2 by day 7 or 13 luteal cell suspensions (P<0.05). It is concluded that adenosine enhances the steroidogenic response by functional bovine luteal cells to hCG, PGE1 or PGE2 and that adenosine reduces the luteolytic response to PGF(subscript 2α) by gonadotropin-stimulated bovine luteal cells in vitro.