Inhibition of Testosterone Secretion by S-Petasin in Rat Testicular Interstitial Cells

S-petasin, a kind of sesquiterpene ester, is the anti-inflammatory and analgesic component of the butterbur (Petasites hybridus). The clinical benefit of S-petasin is the spasmolytic activity, but its side effects on the reproductive endocrinology are not clear yet. The present study was to explore the effects of S-petasin on the secretion of testosterone in vivo and in vitro. We found that single intravenous injection of S-petasin (1μg/kg) decreased basal plasma testosterone concentration in adult male rats. The enzymatically dispersed rat testicular interstitial cells were incubated with S-petasin (0~4.3×10^(-5)M) in the presence or absence of human chorionic gonadotropin (hCG, 0.05IU/ml), forskolin (adenylyl cyclase activator, 10^(-5)M), and androstenedione (testosterone biosynthesis precursor, 10^(-5)M) at 34℃ for 1h. The concentrations of testosterone in the incubation medium were measured by radioimmunoassay. S-petasin at 4.3×10^(-7)M was effective to reduce the basal and hCG-stimulated release of testosterone in rat testicular interstitial cells. The stimulatory effects of testosterone secretion induced by forskolin and androstenedione were significantly reduced by S-petasin at 4.3×10^(-5)M and 4.3×10^(-6)M, respectively. These results suggest that S-petasin inhibits the production of testosterone in rat testicular interstitial cells in part through diminishing the activities of adenylyl cyclase and 17-ketosteroid reductase.

並列關鍵字

testosterone ； S-petasin ； rat ； testicular interstitial cells

被引用紀錄

Liu, Y. A. (2014). 人體腸道組織之三維影像研究 [doctoral dissertation, National Tsing Hua University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0016-2912201413510882

國際替代計量

Inhibition of Testosterone Secretion by S-Petasin in Rat Testicular Interstitial Cells

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