- 期刊
IgA Immune Complex Blunts the Contraction of Cultured Mesangial Cells through the Inhibition of Protein Kinase C and Intracellular Calcium
並列摘要
The effects of IgA immune complex (IgA-IC) on the contractile function of cultured mesangial cells were measured by the changes in planar surface area in response to treatment with agonists. Incubation of mesangial cells with IgA-IC for 24 hours significantly decreased the contractile responses to angiotensin Ⅱ(10^(-6) M) and phorbol 12-myristate 13-acetate (PMA, 10^(-6) M). Pretreatment of mesangial cells with the protein kinase C (PKC) inhibitor, chelerythrine (10^(-6) M), eliminated the difference in contractile responses to angiotensin II or PMA between the control and IgA-IC groups indicating IgA-IC may inhibit the activity of PKC. The contractile responses to ionomycin were not significantly different between IgA-IC treated and control mesangial cells, suggesting that the contractile machinery is not impaired by IgA-IC. Intracellular calcium, [Ca(supscript +2)](subscript i) measured by changes in fura-2 level in response to ATP or bradykinin, was significantly inhibited in IgA-IC treated mesangial cells, compared to control cells. In contrast, treatment with thapsigargin did not result in significant differences in [Ca(supscript +2)] (subscript i) between IgA-IC and control mesangial cells, suggesting that a negligible role of endoplasmic reticulum in the effects of IgA-IC. Using PKC specific antibodies, IgA-IC significantly increased the particulate fraction of PKC-l of mesangial cells to 141±13% of control, without significantly changing the protein content of PKC-α, -δ and –λ in the cytosolic and particulate fractions. In summary, IgA-IC inhibits the contractile responses of cultured mesangial cells to agonists by inhibiting the activation of PKC and [Ca(superscript 2+)](subscript i).
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延伸閱讀
- Peng, Y. S., Hung, K. Y., Hung, J. Q., Chen, C. T., Lee, P. H., Tsai, T. J., & Hsieh, B. S. (2000). Dipyridamole Inhibits PDGF-Stimulated Human Peritoneal Mesothelial Cell Proliferation: Effects on Cyclin Kinase Inhibitors (CKI). Acta Nephrologica, 14(4), 285-285. https://www.airitilibrary.com/Article/Detail?DocID=10131671-200010-14-4-285-285-o
- 卓建宇（2006）。Plumbagin induces apoptosis and cell cycle arrest inA549 cells through p53 accumulation via JNK-mediated phosphorylation at Serine 15 in vitro and in vivo〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-1606200619323800
- Iyoda, T., & Fukai, F. (2012). Modulation of Tumor Cell Survival, Proliferation, and Differentiation by the Peptide Derived from Tenascin-C: Implication of β1-Integrin Activation. International Journal of Cell Biology, 2012(), 736-745. https://doi.org/10.1155/2012/647594
- 林子暄（2018）。Study the role of spleen tyrosine kinase(Syk) in regulating polymeric immunoglobulin receptor(pIgR) expression in intestinal epithelial cell(IEC)〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201803361
- 廖志裕（2006）。Protein kinase C δ plays a critical role in bFGF-induced urokinase-type plasminogen activator production in human fibroblasts〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2006.01947