Neuroprotective Effects of Hydroxyethylpuerarin against Focal Cerebral Ischemia-Reperfusion in Rats

Our present study was performed to investigate whether hydroxyethylpuerarin (HEP) has a neuroprotective effect on brain injury after focal cerebral ischemia/reperfusion by middle cerebral artery occlusion (MCAO) in adult male Wistar rats. Animals were subjected to one hour of middle cerebral artery occlusion and 48 hours of reperfusion with the pretreatment of drugs (HEP 15, 30, 60mg/kg or nimodipine 0.4mg/kg i. v.) or vehicle. The behavioral tests were used to evaluate the damage to central nervous system. The percentage of brain infarct area was assessed in the brain slices stained with 2% solution of 2, 3, 5-triphenyl tetrazolium chloride (TTC). The pathologic histological changes were observed by H&E staining and the occurrence of apoptosis was determined by flow cytometry. The results showed that pretreatment with HEP at doses of 15, 30, 60mg/kg exhibited significant neuroprotective effects on rats against focal cerebral ischemia-reperfusion injury by markedly decreasing neurological deficit scores and the percentage of infarct area, reducing necrosis and apoptosis of neurons. All these findings suggest that HEP might provide neuroprotection against focal cerebral ischemia/reperfusion injury probably through its antioxidant and anti-inflammatory property.

並列關鍵字

hydroxyethylpuerarin ； cerebral ischemia/reperfusion injury ； neuroprotection ； anti-oxidation ； anti-inflammation

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被引用紀錄

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延伸閱讀

Lou, H. Y., Zhang, X. M., Wei, X. B., Wang, R. X., & Sun, X. (2004). Anti-inflammatory Effect of Hydroxyethylpuerarin on Focal Brain Ischemia/Reperfusion Injury in Rats. The Chinese Journal of Physiology, 47(4), 197-201. https://www.airitilibrary.com/Article/Detail?DocID=03044920-200412-47-4-197-201-a
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邱肇祥（2013）。Neuroprotective effect of natural compounds against Aβ1-42 –induced toxicity in rat cortical neurons〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2013.01404
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國際替代計量

Neuroprotective Effects of Hydroxyethylpuerarin against Focal Cerebral Ischemia-Reperfusion in Rats

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