Effects of Scutellarin on Apoptosis Induced by Cobalt Chloride in PC12 Cells

Li-Xiang Wang ； Ji-Ping Zeng ； Xin-Bing Wei ； Fu-Wu Wang ； Zhao-Ping Liu ； Xiu-Mei Zhang

《The Chinese Journal of Physiology》 50卷6期 (2007/12) Pp. 301-307

並列摘要

The present study investigated the protective effects of scutellarin on cobalt chloride (CoCl2)-induced apoptosis in PC12 cells. Incubation of PC12 cells with 500μM CoCl2 for 24h resulted in significant apoptosis as evaluated by the crystal violet, electron microscopy and flow cytometry assays. The increase of caspase-3 activity, decrease of Bcl-X(subscript L) expression, phosphorylation of p38 mitogen-activated protein kinase (MAPK) and accumulation of intracellular reactive oxygen species (ROS) were also seen in CoCl2-treated PC12 cells. Scutellarin at 0.1, 1 and 10μM significantly protected against the apoptotic cell death induced by CoCl2. Scutellarin decreased caspase-3 activity, increased Bcl-X(subscript L) expression, inhibited p38 phosphorylation and attenuated ROS production. These results demonstrate that scutellarin can protect PC12 cells from cobalt chloride induced apoptosis by scavenging ROS, inhibiting p38 phosphorylation, up-regulating Bcl-X(subscript L) expression and decreasing caspase-3 activity, and may reduce the cellular damage in pathological conditions associated with hypoxia-mediated neuronal injury.

並列關鍵字

scutellarin ； flavone glycoside ； PC12 cells ； cobalt chloride ； apoptosis ； reactive oxygen species ； caspase-3 ； p38 MAPK

參考文獻

Adrends, M.J.,Morris, R.G.,Eillie, S.H.(1990).Apoptosis: the role of the endonuclease.Am. J. Pathol..136,593-608.

Blomgren, K.,Zhu, C.,Wang, X.,Karlsson, J.O.,Leverin, A.L.,Brhr, B.A.,Mallard, C.,Hagberg, H.(2001).Synergistic activation of caspase-3 by m-calpain after neonatal hypoxia-ischemia: a mechanism of "pathological apoptosis"?.J. Biol. Chem..276,10191-10198.

Bossenmeyer-Pourie, C.,Koziel, V.,Daval, J.L.(1999).CPP32/caspase-3-like proteases in hypoxia-induced apoptosis in developing brain neurons.Brain Res. Mol. Brain Res.71,225-237.

Chae, H.J.,Kim, S.C.,Han, K.S.,Chae, S.W.,An N.H.,Kim, H.M.,Kim, H.H.,Lee, Z.H.,Kim, H.R.(2001).Hypoxia induces apoptosis by caspase activation accompanying cytochrome C release from mitochondria in MC3T3E1 osteoblasts. P38 MAPK is related in hypoxia-induced apoptosis.Immunopharm. Immunot..23,133-152.

Chandel, N.S.,Maltepe, E.,Goldwasser, E.,Mathieu, C.E.,Simon, M.C.,Schumacker, P.T.(1998).Mitochondrial reactive oxygen species trigger hypoxia-induced transcription.Proc. Natl. Acad. Sci. USA.95,11715-11720.