- 期刊
Effect of m-3M3FBS on Ca(superscript 2+) Movement in PC3 Human Prostate Cancer Cells
並列摘要
The effect of 2, 4, 6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS), a presumed phospholipase C activator, on cytosolic free Ca(superscript 2+) concentrations ([Ca(superscript 2+)](subscript i)) in PC3 human prostate cancer cells is unclear. This study explored whether m-3M3FBS changed basal [Ca(superscript 2+)](subscript i) levels in suspended PC3 cells by using fura-2 as a Ca(superscript 2+)-sensitive fluorescent dye. M-3M3FBS at concentrations between 10-50μM increased [Ca(superscript 2+)](subscript i) in a concentration-dependent manner. The Ca(superscript 2+) signal was reduced by 60% by removing extracellular Ca(superscript 2+). M-3M3FBS-induced Ca(superscript 2+) influx was inhibited by the store-operated Ca(superscript 2+) channel blockers nifedipine, econazole and SK&F96365, and by the phospholipase A2 inhibitor aristolochic acid. In Ca(superscript 2+)-free medium, 30μM m-3M3FBS pretreatment greatly inhibited the [Ca(superscript 2+)](subscript i) rise induced by the endoplasmic reticulum Ca(superscript 2+) pump inhibitor thapsigargin or BHQ. Conversely, pretreatment with thapsigargin, BHQ or cyclopiazonic acid reduced the major part of m-3M3FBS-induced [Ca(superscript 2+)](subscript i) rise. Inhibition of phospholipase C with U73122 did not much alter m-3M3FBS-induced [Ca(superscript 2+)](subscript i) rise. Collectively, in PC3 cells, m-3M3FBS induced [Ca(superscript 2+)+](subscript i) rises by causing phospholipase C-independent Ca(superscript 2+) release from the endoplasmic reticulum and Ca(superscript 2+) influx via store-operated Ca(superscript 2+) channels.
參考文獻
延伸閱讀
- Lee, H. H., Chou, C. T., Liang, W. Z., Chen, W. C., Wang, J. L., Yeh, J. H., Kuo, C. C., Shieh, P., Kuo, D. H., Chen, F. A., & Jan, C. R. (2013). Ca^(2+) Movement Induced by Deltamethrin in PC3 Human Prostate Cancer Cells. The Chinese Journal of Physiology, (), 1-8. https://doi.org/10.4077/CJP.2016.BAE386
- Lee, H. H., Chou, C. T., Liang, W. Z., Chen, W. C., Wang, J. L., Yeh, J. H., Kuo, C. C., Shieh, P., Kuo, D. H., Chen, F. A., & Jan, C. R. (2016). Ca^(2+) Movement Induced by Deltamethrin in PC3 Human Prostate Cancer Cells. The Chinese Journal of Physiology, 59(3), 148-155. https://doi.org/10.4077/CJP.2016.BAE386
- Fang, Y. C., Chou, C. T., Liang, W. Z., Chang, H., Hsu, S. S., Tsai, J. Y., Liao, W. C., Chen, I. S., Liu, Y. Y., Chang, P. M., Yu, C. C., Kuo, C. C., Chen, F. A., & Jan, C. R. (2016). Effect of 2,4-Dimethylphenol on [Ca^(2+)]_i and Viability in PC3 Human Prostate Cancer Cells. Adaptive Medicine, 8(3), 128-135. https://doi.org/10.4247/AM.2016.ABG154
- Lai, Z. R., Chou, C. T., Liu, S. I., Liang, W. Z., Huang, J. K., & Jan, C. R. (2013). Effect of BayK 8644 on [Ca^(2+)]i and Viability in PC3 Human Prostate Cancer Cells. The Chinese Journal of Physiology, 56(6), 318-325. https://doi.org/10.4077/CJP.2013.BAB161
- Liu, S. I., Lin, K. L., Lu, T., Lu, Y. C., Hsu, S. S., Tsai, J. Y., Liao, W. C., Huang, F. D., Chi, C. C., Liang, W. Z., Tseng, L. L., Chiang, A. J., & Jan, C. R. (2013). M-3M3FBS-Induced Ca^(2+) Movement and Apoptosis in HA59T Human Hepatoma Cells. The Chinese Journal of Physiology, 56(1), 26-35. https://doi.org/10.4077/CJP.2013.BAA091