Role of Estrogen Receptor in Astringinin-Mediated Attenuation of Intestinal Injury after Trauma-Hemorrhage

Huang-Ping Yu ； Fu-Chao Liu ； Ying-Tung Lau

《The Chinese Journal of Physiology》 55卷4期 (2012/08) Pp. 229-235

https://doi.org/10.4077/CJP.2012.BAA023

並列摘要

Astringinin protects against organ injury caused by trauma-hemorrhage though the mechanism remains unknown. We hypothesize that astringinin administration in males following trauma-hemorrhage protects against intestinal injury through an estrogen receptor-dependent pathway. To test this hypothesis, male Sprague-Dawley rats were subject to trauma-hemorrhage (mean blood pressure: 40 mmHg for 90 min) followed by fluid resuscitation. Animals were pretreated with an estrogen receptor antagonist (ICI 182,780) 30 min before vehicle or astringinin (0.3 mg/kg) administration, followed by resuscitation, and the treated rats were killed 24 h thereafter. Intestinal wet/dry weight ratio, myeloperoxidase (MPO) activity, and the levels of interleukin (IL)-6, intercellular adhesion molecule (ICAM)-1, cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-3 were measured. Traumahemorrhage led to an increase in intestinal wet/dry weight ratio, MPO activity, histological damage and also increases in the levels of IL-6, ICAM-1, CINC-1 and CINC-3. Administration of astringinin improved all of the above parameters. Administration of ICI 182,780 with astringinin abolished the astringinin-mediated improvement of the above parameters. These results suggest that the estrogen receptor-dependent pathway likely plays a critical role in mediating the salutary effects of astringinin on shock-induced intestinal injury.

並列關鍵字

shock ； estrogen receptor ； intestine ； chemokine ； adhesion molecule ； cytokine

參考文獻

Caputo, F.J.,Rupani, B.,Watkins, A.C.,Barlos, D.,Vega, D.,Senthil, M.,Deitch, E.A.(2007).Pancreatic duct ligation abrogates the trauma hemorrhage-induced gut barrier failure and the subsequent production of biologically active intestinal lymph.Shock.28,441-446.

Choudhry, M.A.,Schwacha, M.G.,Hubbard, W.J.,Kerby, J.D.,Rue, L.W.,Bland, K.I.,Chaudry, I.H.(2005).Gender differences in acute response to trauma-hemorrhage.Shock.24(Suppl 1),101-106.

Dang, O.,Navarro, L.,David, M.(2004).Inhibition of lipopolysaccharide-induced interferon regulatory factor 3 activation and protection from septic shock by hydroxystilbenes.Shock.21,470-475.

Huang, Y.S.,Liu, F.C.,Li, A.H.,Lau, Y.T.,Yu, H.P.(2011).Astringinin-mediated attenuation of the hepatic injury following trauma-hemorrhage.Chinese J. Physiol..54,183-189.

Jin, C.Y.,Moon, D.O.,Lee, K.J.,Kim, M.O.,Lee, J.D.,Choi, Y.H.,Park, Y.M.,Kim, G.Y.(2006).Piceatannol attenuates lipopolysaccharide-induced NF-κB activation and NF-κB-related proinflammatory mediators in BV2 microglia.Pharmacol. Res..54,461-467.