A Viscometric Method to Study the Effects of Hematocrit of Blood and Different Surfaces of Biomaterials on Blood Clot Formation

以黏度量測法研究血比容及不同生醫材料表面對血栓形成的影響

王毓萱(Yu-Hsuan Wang) ； 鐘次文(Tze-Wen Chung) ； 賴君義(Juin-Yih Lai) ； 徐仁基(Jen-Ji Hsu) ； 林永生(Yeong-Shang Lin)

《Journal of the Chinese Institute of Chemical Engineers》 31卷1期 (2000/01) Pp. 27-32

https://doi.org/10.6967/JCICE.200001.0027

摘要

血液因凝固現象所造成的黏度上升可被黏度儀所測出。以此原理，我們用cone/plate黏度儀以剪切率90sec-1及凝固啟始時間(t0)及血栓形成強度(dη/dt)等兩參數來描述血液凝固的現象。另外，我們以改變血比容及在plate上不同的表面材料等兩種因子，來測上述參數的敏感性。結果顯示，高血比容的樣本易縮短t0，但會增強dη/dt；PU-PEG薄膜可大幅延長t0的發生，而PU可大大的減低dη/dt。PMMA可有效的延長t0，但不能有效的減低dη/dt。由上述的研究，我們認為黏度量測法及兩種參數可用來偵測上述因子所造成血液凝固變化的程序；同時建議可應用於材料的動態測試。

並列摘要

The increasing viscosity of blood during blood coagulation and clot formnation process could be detected by a viscometric method. To characterize the above-mentioned process by a cone/plate viscometer, we defined initial time of coagulation, to, and the intensity of clot formation, dη/dt, as parameters to detect the process at a shear rate of 90 see-1, at which shear stress was about 4. 5 dyne/cm2. Moreover, to test the sensitivity of the method and parameters, we investigated whether they could detect the changes of different hematocrit (Hct) of blood samples, and material surfaces of plate during coagulation process. The results showed that high Hct of blood samples would shorten to but intensify dη/dt. Among those tested materials, PU grafted with PEG (PU-PEG) film could prolong much longer and PU film could dramatically reduce the dη/dt values. PMMA could prolong long but it was not so effective to reduce dη/dt value. Through the study, we suggested that the method and parameters are sensitive to the changes of the above-mentioned factors for detecting blood coagulation process. They can be further applied for studying blood coagulation process or for dynamic testing the blood compatibility of different materials in vitro.

並列關鍵字

Viscometric Testing ； Hematocrit ； Biomaterials ； Blood Clot Formation