- 期刊
Production of Nuclease in Fed-Batch Culture of Recombinant Escherichia coli
以基因重組大腸桿菌行饋料批次醱酵生產核酸分解酵素
摘要
本文探討在以基因重組大腸桿菌生產Vibrio vulnificus核酸分解酵素之醱酵中，促進酵素生產之策略。在批次醱酵中，使用複合培養基可得最大的核酸分解酵素產量3500 U/mL；此複合培養基之特點為含有高濃度的酵母萃出液、蛋白胴、鎂離子。在醱酵過程中，醋酸的生成是核酸分解酵素生產的一個不利因素。控制溶氧濃度葡萄糖進料都可抑制醋酸的生成，因而促進核酸分解酵素之生產；其中，控制溶氧濃度是比較有效之策略。使用控制pH值之饋料批次醱酵，並在醱酵過程中維特溶氧濃度在15%飽和值之上，且間斷地補充醱酵液之養分，核酸分解酵素產量可達12000 U/mL。
關鍵字
無資料並列摘要
Strategies for enhancing the production of Vibrio vulnificus nuclease using a recombinant Escherichia coli were explored. Using a batch culture, a maximum nuclease yield of 3500 U/mL was obtained with a complex medium characterized by high concentrations of yeast extract, tryptone and magnesium ion. The formation of acetate during fermentation was considered to be detrimental to the nuclease production. Both dissolved oxygen (DO) control and glucose feeding could suppress acetate formation, thus enhancing nuclease production, where the former was found to be more effective. Using a pH-stat fed-batch culture with the DO level maintained above 15% air saturation and with intermittent addition of nutrients, a nuclease yield of 12000 U/mL was achieved.
延伸閱讀
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