Improved Cultivation of the Indigenous Acidithibacillus thiooxidans BC1 by a Fed-Batch Process

以饋料批次培養高濃度本土性硫氧化硫化桿菌來增加硫酸產量

劉宣良(Hsuan-Liang Liu) ； 陳博彥(Bor-Yann Chen) ； 藍彥文(Yann-Wen Lan) ； 鄭陽助(Yang-Chu Cheng)

《Journal of the Chinese Institute of Chemical Engineers》 35卷2期 (2004/03) Pp. 195-201

https://doi.org/10.6967/JCICE.200403.0195

摘要

本研究設計一簡單之饋料批次程序進行本土性硫氧化硫化桿菌之高濃度培養來增加硫酸的產量。因爲在酸性條件下的代謝中間產物亞硫酸會對此菌種生長產生抑制作用，故pH值的調控對此菌種的生長有重要的影響。除此之外，固態硫粉是此菌種生長唯一的能量來源，故連續饋料固態硫粉的批次程序證實可以避免基質的不足及抑制效應，而抑制效應主要導因於固態硫粉累積於氣液介面。以此饋料批次程序所生產的細胞濃度及硫酸產量比傳統的批次程序分別高出2.16及3.25倍。最佳的饋料批次程序則設定為：每當pH值自2.0調升為4.0時，添加2g/L的固態硫粉基質於液態培養基中。

並列摘要

In this study, a simple fed-batch process was developed for high cell density culture of the indigenous Acidithiobacillus thiooxidans BC1 for enhanced production of sulfuric acid. Since the inhibitory effect of the metabolic intermediate, sulfite, occurs in the acidic condition, pH control is important in the growth of this microorganism. In addition, elemental sulfur is the sole energy source for the indigenous A. thiooxidans BC1, a fed-batch process including the intermittent feeding of elemental sulfur was proved to avoid the effect of substrate limitation and inhibition, which was resulted from the sulfur accumulation in the air-liquid interface. The maximal cell density and sulfuric acid yield obtained in this study were 2.16 and 3.25 times, respectively, higher than those obtained from the conventional batch process. The optimal operation condition for the fed-batch process was setas: intermittent feeding of additional 2g/L elemental sulfur at the same time when the pH value was adjusted from 2.0 to 4.0.

並列關鍵字

Fed-batch ； High cell density ； Acidithiobacillus thiooxidans ； Sulfuric acid ； Elemental sulfur ； Sulfite