Biodegradable Polymeric Nanoparticles Bearing Stealth PEG Shell and Lipophilic Polyester Core

具有隱藏性聚乙二醇表層與疏水性聚酯核心的生物可分解性高分子奈米顆粒

謝明發(Ming-Fa Hsieh) ； 林才祐(Tsai-Yu Lin) ； 高榮駿(Rung-Jiun Gau) ； 張學曾(Hsuen-Tseng Chang) ； 羅又伶(You-Ling Lo) ； 賴建勳(Chien-Hsun Lai)

《Journal of the Chinese Institute of Chemical Engineers》 36卷6期 (2005/11) Pp. 609-615

https://doi.org/10.6967/JCICE.200511.0609

摘要

聚乙二醇化的顆粒透過靜脈注射後在血液循環中被認為可避免巨噬細胞吞噬，因此，搭載藥物的顆粒可以延長在血流中的循環時間。在本研究中，我們使用聚乙二醇－PCL團聯共聚合物形成奈米顆粒以避免巨噬細胞攻擊我們使用MTT方法評估團聯共聚合物自我組裝成的奈米顆粒對細胞的毒性。流式細胞儀則用來分析高分子奈米顆粒與巨噬細胞株(RAW264.7)的交互作用。與PEG-PCL交互作用後發現巨噬細胞的顆粒性並未顯著增加，但在PCL-PAA（非聚乙醇化高分子）交互作用下細胞顆粒性明顯增加。本研究中的初步的結果顯示巨噬細胞並未被PEG-PCL奈米顆粒所活化。

並列摘要

PEGylated particles are considered to be macrophage-resistant in blood circulation following intravenous injection. Therefore, drug-loaded particles in blood stream can extend its lifetime. In this study, we utilized PEG-PCL block copolymers designed to avoid attacking from macrophages. We used MTT assay to evaluate the cytotoxicity of nanoparticles formed by self-assembly of the block copolymers. The flow cytometry was utilized to analyze the interaction of the polymeric nanoparticles with a macrophage cell line (RAW264.7). It was found that the cell granularity did not change significantly in PEG-PCL block copolymer yet increased substantially in PCL-PAA (non PEG grafted copolymer). The preliminary results in this study suggested that the macrophage cells were not activated by PEG-PCL nanoparticles.

並列關鍵字

Drug delivery systems DDS ； Macrophage ； Stealth ； Flow cytometry