Characterization and Application of Carica papaya Lipase to the Dynamic Kinetic Resolution of (R,S)-Naproxen Thioester

木瓜脂肪分解酵素之特性分析與應用至(R,S)-Naproxen硫酯之動態動力分割

吳意珣(I-Son Ng) ； 蔡少偉(Shau-Wei Tsai)

《Journal of the Chinese Institute of Chemical Engineers》 37卷4期 (2006/07) Pp. 375-382

https://doi.org/10.6967/JCICE.200607.0375

摘要

以橄欖油為基質，pH、溫度與金屬離子為參數，本研究首先比較部分純化木瓜脂肪分解酵素(pCPL)與其粗酵素(CPL)於水溶液中之水解行為。其次於(R,S)-naproxen硫酯於含飽和水有機溶劑之水解分割為模式，發現pCPL比CPL與Candida rugosa脂肪分解酵素(CRL)具有較佳之酵素活性、熱穩定性、立體選擇性、回收再使用性。最後以pCPL催化(R,S)-naproxen硫酯之動態動力分割，發現所添加三辛基胺消旋觸媒之濃度對(R)-與(S)-硫酯之動力學參數有重大影響。

並列摘要

With pH, temperature and metal ions as the parameters, the lipolysis of olive oil in buffers via a partially purified Carica papaya lipase (pCPL) was investigated and compared with that via crude preparation (CPL). The lipase-catalyzed hydrolysis of (R,S)-naproxen thioester in water-saturated organic solvents was then employed as the other model system, in which pCPL was found to be superior to CPL and Candida rugosa lipase (CRL) when the enzyme activity, thermal stability, enantioselectivty and reuse of the recovered lipase was considered. Moreover, in the pCPL-catalyzed dynamic kinetic resolution of (R,S)-naproxen thioester, the concentration of trioctylamine added as a racemization catalyst was found to have profound effects on the kinetic constants for (R)-and (S)-thioester.

並列關鍵字

Partially purified Carica papaya lipase ； Dynamic kinetic resolution ； R,S-naproxen thioester ； Trioctylamine