In this study, the influence of substrate-limited media (nitrogen or phosphorus) on Ralstonia eutropha (ATCC 17699) growth and poly-hydroxybutyrate (PHB) production in a batch fermenter at various temperatures (26, 30 and 35℃) was explored. The intracellular PHB yields of bacteria using different limiting substrates were compared. The Ralstonia eutropha had a significantly shortened generation time at higher temperatures (between 26 and 35℃), regardless of the limiting media used. In both limiting media, the PHB accumulation was the highest when the temperature was 35℃. The highest ratios of PHB to biomass in the nitrogen-limiting medium and in the phosphorus-limiting medium were 66.1% and 43.1%, respectively.