Biohydrogen Production Using Suspended and Immobilized Mixed Microflora

以懸浮與固定化混合菌群進行生物醱酵產氫

吳坤哲(Ken-Jer Wu) ； 張嘉修(Jo-Shu Chang) ； 張瓊妃(Chiung-Fei Chang)

《Journal of the Chinese Institute of Chemical Engineers》 37卷6期 (2006/11) Pp. 545-550

https://doi.org/10.6967/JCICE.200611.0545

摘要

本研究利用蔗糖為碳源，以懸浮和固定化厭氧污泥進行批次醱酵產氫試驗。藉由一系列的固定化方法來測試產氫材料的效率與穩定性。結果顯示以複合材料（藻酸鈣＋氧化鈦＋幾丁聚醣）製備之固定化細胞產氫效果最佳，於35℃和蔗糖濃度為20 g COD•L^(-1)時，可得產氫速率為21.3 mmol•L^(-1)•h^(-1)，此速率為懸浮細胞(6.8 mmol•L^(-1)•h^(-1))的三倍多。在此批次產氫試驗中，蔗糖轉化率可達85%以上，氣相產物只含氫氣和二氧化碳，主要的液相代謝產物為丁酸、乙酸、丙酸和少量的乙醇。於懸浮細胞批次試驗中，添加微量的Ca(上标 2+)和Ni(上标 2+)離子，可讓產氫速率從6.8 mmol•L^(-1)•h^(-1)分別增至9.3 mmol•L^(-1)•h^(-1)和8.7 mmol•L^(-1)•h^(-1)。此外，本研究結果亦發現，添加氧化鈦之共固定化細胞材質可有效地增進生物產氫效率。

並列摘要

Suspended and immobilized anaerobic sludge were utilized to produce hydrogen gas in batch reactors using sucrose as the sole carbon substrate. A variety of immobilization matrices and methods were examined for their suitability to make immobilized H2 producers. The results show that cells immobilized by calcium alginate matrix supplemented with chitosan and titanium oxide carriers (CA+CH+TiO2) were the most effective in H2 production, giving a H2 production rate of 21.3 mmol•L^(-1)•h^(-1) (at 35℃ and 20 g COD•L^(-1) of sucrose), which was over three-fold of that obtained from suspended cells (6.8 mmol•L^(-1)•h^(-1)). The sucrose conversion was essentially 85% or higher in all runs, and the biogas consisted of only H2 and CO2. The major soluble metabolites were butyric acid, acetic acid, and propionic acid, while a small amount of ethanol was also produced. Addition of trace metal salts (Ca(superscript 2+) and Ni(superscript 2+)) appeared to enhance the H2 producing performance for the suspended culture, elevating H2 production rate from 6.8 to 9.3 and 8.7 mmol•L^(-1)•h^(-1), respectively. Moreover, the immobilization matrix containing TiO2 seemed to result in better H2 production in general.

並列關鍵字

Biohydrogen production ； Immobilized cell ； Alginate ； Composite material ； Anaerobic sludge