並列摘要
Alpha-amylase was purified from fresh potato tuber homogenate by extraction at pH 5.0 with acetate buffer followed by glycogen precipitation and chromatography on Sephadex G75. The purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis. From gel filtration, the molecular weight was determined as 42℃. Activation energy between 26℃-42℃ was 8200 calories per mole and the Km was 5.2X10^(-3) g/ml on modified soluble starch.
並列關鍵字
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