- 期刊
Preliminary Experiments on Freeze Preservation of Sugarcane Cells
並列摘要
A preliminary investigation on freeze preservation of sugarcane cell cultures indicated that sugarcane cultures frozen at a rate of 1℃/min to -100℃ after treating with 10% (v/v) glycerol +5% (v/v) dimethylsulfoxide (DMSO), and thawed in a water bath at 37℃, yielded up to 30% viable cells. 10% glycerol was found to be more effective cryoprotectant for sugarcane cells than 5% DMSO. Rapid thawing in a water bath at 37℃ gave the highest viability of sugarcane cells recovered following freezing. Frozen sugarcane cells stored in the liquid nitrogen refrigerator, up to 4 months, indicate that no decrease in cell viability was observed after thawing as compared with control.
並列關鍵字
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延伸閱讀
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