- 期刊
Use of Random Amplified Polymorphic DNAs Markers to Distinguish Temperature Isolates of Aspergillus fumigatus of Taiwan
利用隨機複製DNA標誌分析台灣產煙麴黴之不同溫度品系
並列摘要
Four temperature isolates of Aspergillus fumigatus Fres. from Taiwan and six isolates from several culture collection centers (CBS, ATCC and IMI) were analyzed by random amplified polymorphic DNAs (RAPDs). Ten oligonucleotide decamers were used to generate selective markers from genomic DNAs and five primers gave adequate discrimination between isolates. All isolates were classified into four groups by RAPDs that consistent to their physiological characteristics. The results indicate that RAPDs analysis is useful to assess the genetic diversity and intraspecies temperature variation among different temperature strains of Aspergillus fumigatus.
延伸閱讀
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