Inorganic Carbon Utilization of the Freshwater Red Alga Compsopogon coeruleus (Balbis) Montagne (Compsopogonaceae, Rhodophyta) Evaluated by in situ Measurement of Chlorophyll Fluorescence

利用葉綠素螢光分析法實地研究淡水紅藻Compsopogon coeruleus（Balbis）Montagne無機碳使用情形

劉少倫(Shao-Lun Liu) ； 汪良奇(Liang-Chi Wang) ； 王瑋龍(Wei-Lung Wang)

《TAIWANIA》 49卷3期 (2004/09) Pp. 209-217

https://doi.org/10.6165/tai.2004.49(3).209

摘要

To explore the inorganic carbon utilization of the freshwater red alga Compsopogon coeruleus, photosynthetic rates in response to increasing of bicarbonate concentration, the addition of alkaline HEPES buffer (pH 8.8), acid HEPES buffer (pH 4.0) and the extracellular carbonic anhydrase inhibitor (acetazolamide, AZ), respectively, were examined in situ by using a submersible pulse amplitude modulated (PAM) fluorometer. Among the treatments, adding acid HEPES buffer significantly reduced photosynthetic rates of the alga, while others showed no effect. Accordingly, we concluded that C. coeruleus had less or no inorganic carbon (Ci) limitation in its natural habitat. The alga might have higher affinity for bicarbonate and directly uptake bicarbonate as main Ci source without the aid of extracellular carbonic anhydrase.

關鍵字

Acetazolamide ； 碳酸酣酶 ； 螢光分析儀 ； 無機碳 ； HEPES ； 飽和性光脈衝 ； 光合作用

並列摘要

To explore the inorganic carbon utilization of the freshwater red alga Compsopogon coeruleus, photosynthetic rates in response to increasing of bicarbonate concentration, the addition of alkaline HEPES buffer (pH 8.8), acid HEPES buffer (pH 4.0) and the extracellular carbonic anhydrase inhibitor (acetazolamide, AZ), respectively, were examined in situ by using a submersible pulse amplitude modulated (PAM) fluorometer. Among the treatments, adding acid HEPES buffer significantly reduced photosynthetic rates of the alga, while others showed no effect. Accordingly, we concluded that C. coeruleus had less or no inorganic carbon (Ci) limitation in its natural habitat. The alga might have higher affinity for bicarbonate and directly uptake bicarbonate as main Ci source without the aid of extracellular carbonic anhydrase.

並列關鍵字

Acetazolamide ； Carbonic anhydrase ； Fluorometer ； Inorganic carbon ； HEPES ； PAM ； Photosynthesis