Aspidistra daqingshanensis (Asparagaceae) is described and illustrated as a new species from Longzhou County, Guangxi, China. It is morphologically similar to Aspidistra fasciaria G.Z. Li but differs in its leaf blade wider to 4-8 cm, distal margin denticulate, perianth lobes broadly ovate, yellow, stigma white, slightly recurved at apex. A somatic chromosome count of 2n = 38 and a karyotype formula 2n =22m + 10sm + 6st were determined for A. daqingshanensis. The new species is currently only known from the type locality in southwest Guangxi, near the border with northern Vietnam.