Sulfuric acid used in the wet digestion of plant sample puts some limit to the determination of calcium. According to the method described here, as high as 4% of the calcium in plant sample can be safely determined. The sulfuric acid can also be used for partial seperation of calcium in alcoholic solution, which makes the magnesium determination more accurate when the sample contains high amount of calcium. Alcohol solution of 50% concentration was found satisfactory for this purpose without removal of alcohol before titration.