蝴蝶蘭禁挿組織培養之研究

Studies on the Clonal Propaganion of Phalaenopsis by Leaf Tissue Culture

林瑞松(Ruey-Song Lin)

《中華農業研究》 29卷3期 (1980/09) Pp. 195-201

https://doi.org/10.29951/JARC.198009.0002

摘要

本研究在探討蝴蝶蘭葉片組織培養明確之配方。1．蝴蝶蘭經B5無機靈及有機鹽，myoinosito 1100mg/l，蔗糖30g/l，agar l%，NAA 5mg/l，kinetin 10mg/1的培養基(pH 5.5)誘導，得癒合組織及芽球相似物突起。經在相同培養基作繼代培養可使分化成幼苗。2．葉組織培養中，以心葉形成芽球相似物之能力爲最高，而在上表皮形成率較下表皮形成率爲高。3．由組織切片顯微鏡觀察，芽球相似物之形成是由表皮細胞及其下面細胞分裂而來。同時亦發現維管束鞘附近薄壁細胞，細胞核／細胞質比例大，具有細胞分裂活性。

並列摘要

An investigation of finding the clear and good media were carried out in order to establish the method of clonal propagation by leaf tisue culture in Phalaenopsis.
Calli and protocorm-like bodies (PLB)were induced from leaf tissue Phalaenopsis of in a basal medium containing Gamborg's B5 salts, 100 mg/l of myoinositol, 30 g/l of sucrose, 10 g/l of agar, 5 mg/l of NAA and 10 mg/l of kinetin, the pH was adjusted to 5.5. Plantlets were also induced by subculture in the same medium.
The ability of PLB formation was higher in the first than in the second and third leaves of ths explant of infant seedling. It was obssrved that PLB occured most frequently on the adaxial rather than the abaxial surface of the leaves.
Studies by light microscopy indicated that PLB formed might be deried from cells just below the epidermal layer. This is further demonstrated by the high nucleus/cytoplasm ratio and active cell division of the parenchyma cells in the bundle sheath tissues.