蝴蝶蘭根組識培養之研究

Studies on the Colonal Propagation of Phalaenopsis by Root Culture

林瑞松(Ruey-Song Lin)

《中華農業研究》 30卷2期 (1981/06) Pp. 141-145

https://doi.org/10.29951/JARC.198106.0006

摘要

本研究在探討蝴蝶蘭根組織培養明確之配方。結果摘錄如下：1.蝴蝶蘭根經B5無機鹽及有機鹽，100mg/l myo-inositol，30g/l蔗糖，5mg/l Naphthaleneacetic acid(NAA)，10mg/l 6-furfurylaminopurine(Kinetin)的培養基(pH5.5)誘導，得癒合組織及芽球相似物突起。2.芽球相似物繼代培養於相同配方的固態培養基6個月後形成蝴蝶蘭幼植株。3.芽球相似物僅能在根尖部位形成。4.照光與未照光處理對芽球相似物之誘導均未有顯著之影響。

並列摘要

This paper describes an efficient method of clonal propagation through Phal aenopsis root cultued in vitro. The result as summaried as follows:
Calli and protocorm like bodies (PLB) were induced from root tip by using Gam borg's B5 basal medium with 100mg/l myo-inositol, 30,000mg/l sucrose, 5mg/l NAA and 10mg/l kinetin. The pH was adjusted to 5.5. In similar solid medium, the PLB were then developed into baby phalaenopsis plants in 6 months. The formation ability of PLB was only on root tip. There is no significant variation between light and dark treatment.