Insecticide Resistance Study in Plutella xylostella (L.) Ⅳ. The Activities of Glutathione-S-transferase in the Organophosphorug-resistant Strains

小菜蛾抗藥性之研究　Ⅳ. Glutathione-S-transferase在抗有機磷品系中之變化

鄭尤(Edward Y. Cheng) ； 周桃美(Tao-Mei Chou) ； 高靜華(Ching-Hua Kao)

《中華農業研究》 32卷4期 (1983/12) Pp. 373-378

https://doi.org/10.29951/JARC.198312.0009

摘要

利用在宜蘭所採得之感性品系小菜蛾，在室內誘發抗佈飛松及抗美文松兩有機磷劑品系，再行比較該等品系小菜蛾體內解毒酵素GSH-S-transferase活性之變化。試驗中利用抗百滅靈及抗賽滅寧二品系作對照，結果發現該解毒酵素的確在抗有機磷品系中提高約四倍，幾乎相當於該品系實際增加之抗性指數(Resistant Ratio)，而抗合成除蟲菊精之品系均無此酵素活性提高之現象，顯然GSH-S-transferase係一抗有機磷之專有機制，此結果與國外報導GSH-S-transferase爲家蠅抗有機磷之主要機制結果相符，因此證明此一抗藥機制亦存在於小菜蛾體內。
試驗中並發現，小某蛾體內之GSH-S-transferase-經分離出體外，活性就開始下降，添加EDTA及BSA均不能阻止活性下降之現象及程度，三小時內活性下降接近零點。

並列摘要

The role of glutathione(GSH)-S-transferase as a detoxication mechanism to organophosphorus insecticides was investigated in the diamondback moth, plutella xylostella (L.). The GSH-S-transferase was prepared fromthe susceptible IL-strain, two organophosphorus-resistant strains and two synthetic pyrethroid-resistant strains. The result indicated that the GSI-I-S-transferasa activities in two organophosphurus-resistant strains were three to four times higher than that of the susceptible IL-strain, i.e., the parental strain from which all resistant strains were derived. Both O-methyl and O-ethyl groups on the organophosphorus insecticides can cause GSH-S-transfer ase activity increases in insecticide pressed diamondback moth population. Hence, the GSH-S-transferase detoxication mechanism is quite specific to the organophosphorus insecticides. The study indicated that the glutathione conjugation is an important resistant mechanism to the organophosphorus insecticides in the diamondback moth.