- 期刊
低溫處理及液體培養對水稻花葯癒傷組織形成之效果
The Effects of Cold Shock and Liquid Medium on Callus Formation in Rice Anther Culture
摘要
水稻花葯培養前，將附有劍葉之稻穗經10℃, 7天之低溫處理，可提高花葯callus形成率一倍，花葯接種於培養基後再行低溫處理則無效。低溫處理花葯所得較callus，分化之綠苗，有較多單倍體而較少雙倍體的趨勢。 液體培養不但無益於花葯callus之誘導，且形成之callus分化力極差，故不適宜水稻花葯培養。
關鍵字
無資料並列摘要
Rice culm with flag leaf was pretreated by low temperature at 10℃ for 7 days. Anthers taken from these pretreated flowers were cultured on solid medium. It was found that cold-shocked anthers produced more callus (2-fold) than those without pretreatment. The effect was not significant when pretreatment was done after anthers have been cultured on medium. Callus initiated from cold-shocked anthers produced more haploid plants and less diploid plants than the callus initiated from anthers without pretreatment. Due to the low differentiation ability of callus initiated from anthers cultured in the liquid medium, it is considered that liquid suspension culture is not suitable for rice anther culture.
並列關鍵字
無資料延伸閱讀
- 葉常青、蔡新聲（1988）。高溫處理及培養基成分對水稻花藥培養之效果。中華農業研究，37(3)，250-256。https://doi.org/10.29951/JARC.198809.0003
- 蔡新聲（1981）。菸草、水稻及蘆筍培養花葯褐變與癒傷組織形成之關係。中華農業研究，30(2)，121-131。https://doi.org/10.29951/JARC.198106.0004
- 陳駿季、蔡新聲（1984）。水稻不同次位分蘖、穗位及枝梗間花葯培養癒傷組織之形成。中華農業研究，33(4)，354-362。https://doi.org/10.29951/JARC.198412.0002
- 許永華、游俊明（1985）。藥劑拌種及土壤填加物防治落花生冠腐病之效果。桃園區農業改良場研究報告，(3)，54-62。https://doi.org/10.29567/ZHWHGX.198512.0003
- 卜瑞雄、陳正昌、黃眞生（1978）。Effects of Sucrose Application on Grain Yield in Meiosis and Maturing Stages of the Rice Plant。中華農業研究，27(2)，169-174。https://doi.org/10.29951/JARC.197806.0004