晚香玉花藥培養及其癒合組織再生株之變異

Anther Culture and Somaclonal Varivation of Tuberose (Polianthes tuberosa L.)

紀海珊(H. S. Gi) ； 蔡新聲(H. S. Tsay)

《中華農業研究》 38卷3期 (1989/09) Pp. 346-352

https://doi.org/10.29951/JARC.198909.0007

摘要

晚香玉單瓣栽培種(Polianthes tuberose L. cv ”Single”)單核期的花藥，接種於含有1ppm 2，4-D、3%蔗糖及0.8%洋菜之1/2MS基本鹽類（Na-Fe-EDTA全量）培養基中，可獲得具有胚發生能力之癒合組織(embryogenic callus)。將此癒合組織移植於植物生長調節劑爲0.2ppm NAA及2ppm kinetin之1/2MS基本鹽類培養基，可獲得擬胚體及再生植株。所獲得35株之再生植株，據調查已開花8株之園藝性狀，顯示有花序增長、花蕾數增加、花徑變大、花瓣數變少、外花被呈粉紅色及葉片大小的差異，此變異或可供爲改良晚香玉單瓣種花序短、花蕾數少及切花品質低等缺點之選拔材料。

並列摘要

Anthers of tuberose variety with single-petal flowers was used in this experiment for callus induction and plant regeneration. Anthers with microspores at uninucleate stage were cultured on medium of 1/2 strength MS basic salts (with full strength of Na-Fe-EDTA) supplemented with 1ppm 2, 4-D, 3% sucrose and 0.8% phytagar. The produced embryogenic callus was able to differentiate into embryoids and plantlets after transfering to medium containing 0.2ppm NAA and 2ppm kinetin. A total of 35 plantlets were produced by the procedures described above. Among them, 8 plants grew to bloom showing significant variaton in various floral traits. These included inflorescence length, number of flower buds, flower size and color. It is suggested that the plants produced from tissue culture can be used as a source for varietal improvement.