利用RAPD技術分析桃種原親緣關係

Genetic Relationship Analysis on Peach Germplasm by RAPD

溫英杰(Ien-Chie Wen) ； 許財誠(Tsai-Cheng Chieh)

《中華農業研究》 52卷2期 (2003/06) Pp. 144-152

https://doi.org/10.29951/JARC.200306.0008

摘要

利用RAPD技術對29個桃品種進行遺傳相似度分析，篩選出35條隨機引子共擴增305條條帶，其中多態性條帶229條占75.1%。群集分析結果，29個試驗品種共分為七群，其中來自巴西、美國、華南的桃種原與矮性桃各自聚為一群，華中桃與日本桃種原另成一群，雞珍桃與離核晚桃則各自成為一群，顯示日本桃品種可能源自大陸華中地區。在所分析的品種中，遺傳相似度係數介於0.73-0.93之間，Flordabelle和Flordagrande的遺傳相似度係數達。0.96，顯示兩品種之親緣關係最近，雞珍桃與其餘試驗品種的遣傳相似度係數為0.73，與其他品種的親緣關係最遠。

關鍵字

桃 ； 種原 ； 逢機增殖多型性DNA

並列摘要

The genetic relationships among 29 peach cultivars were analyzed by using RAPD technology. A total of 220 primers were screened for their ability to produce strongly amplified products. Out of 35 primers amplified 305 DNA bands, 229 of which were polymorphic (75.1%). A dendrogram based on the UPGMA cluster analysis was constructed. All tested peach cultivars were divided into seven groups which were Brazilian peach group, American peach group, south China peach group, central China and Japan peach group, dwarf peach group, Li Ho Wan Tao group and Chi Chen Tao group. The results indicated Japan peach varieties may originate from central China. Flordabelle and Flordagrande had 96% similarity indexes, indicating the closest genetic relationship. Otherwise, Chi Chen Tao having 73% similarity indexes showed the farest genetic relationship with other tested peach varieties.

並列關鍵字

Prunus persica ； Germplasm ； RAPD