烏魚精液保存試驗—Ⅱ.稀釋精液及添加抗凍劑對冷凍前之精子活力與授精力之影響

Experiment on Cryogenic Preservation of Grey Mullet Sperm-Ⅱ. Effect of Dilution and Cryoprotective Agents on Prefreeze Motility and Fertility of Mullet Spermatozoa

趙乃賢(Nai-Hsien Chao) ； 陳惠彬(Huei-Pin Chen) ； J. K. Sherman ； 廖一久(I-Chiu Liao)

《臺灣水產學會刊》 3卷1期 (1974/06) Pp. 1-9

https://doi.org/10.29822/JFST.197406.0001

摘要

1973年12月至1974年2月，再度配合東港分所之烏魚人工繁殖試驗，進行探討稀釋精液及添加抗凍劑對冷凍前之烏魚精子活力與授精力之影響，所得結果如下：
(1)本試驗所試之稀釋用抗凍劑最終濃度在8至38%範圍內，DMSO比Glycerin對精子活力具有較大之抑制力。
(2)普遍而言，稀釋液採用海產魚類生理食鹽水添加10% Glycerin組比不添加組，對精子活力具有較大之抑制力。
(3)以海產魚類生理食鹽水添加或不添加Glycerin用以稀釋精液且達1:50，1:100倍數時，1 hr以內，兩者均開始對精子活力有抑制力。
(4)對冷凍前之授精力，添加5%或10%之Glycerin將會減低，然而添加5%或10% DMSO時，將增進其授精力，至於有關之mechanism則有待今後之進一步探討。
(5)以適當倍數之海產魚類生理食鹽水稀釋烏魚精液（如1:5），將有助於增進冷凍前精子的活力及授精力。
(6)冷凍前之平衡時間以1 hr之內為宜，如此可確保冷凍前活力及冷凍又解凍後之授精力。

並列摘要

Investigations on the feasibility of cryopreservation of spermatozoa of the grey mullet (Mugil cephalus Linnaeus) (superscript (1,2)) was continued during the recent spawning season (December1973 through February 1974). The aim of this investigation was to evaluate the possible deleterious effects of dilution and of cryoprotective agents in pretreatment of spermatozoa prior to cooling and freezing.
The experiment analyzed 1) the effect on motility of diluent, dilution, and cryoprotectiveagent, with time at room temperature, and 2) the effect on fertility of dilution with and without cryoprotective agents in probable routine treatment prior to attempts at cryopreservation.
Results indicated the following: 1) Sea water proved unsuitable as a diluter, while Ringer solution for marine fish supported motility very well for about three hours, even in dilutions up to twenty-fold. 2) DMSO (dimethylsulfoxide) proved more toxic, or reduced motility more, than glycerin during exposure of one hour or more, in concentrations of 8 to 38%. 3) Glycernin reduced motility only slightly during a two-hour period in dilutions up to tenfold. 4) Dilution with or without glycerin in dilutions above 1:20, i.e. 1:50 and 1:100, reduced motility usually within one hour. 5) Dilution of semen up to ten-fold with Ringer solution for marine fish not only was compatible with fertility, but also improved upon the fertilizing capacity of undiluted control samples. 6) DMSO did not reduce fertility. In fact, it increased it, even at the 10% level. Glycerin was compatible with fertility but often reduced it, especially at the 10% concentration. 7) While salient results generally were consistent in relative findings, variability between samples and poor performance of germ cells sometimes restricted the number of reproducible observations necessary for more quantitative conclusions.