日本鰻魚(Anguilla japonica)對Edwardsiella anguillimortiferum & Aeromonas hydrophila抗原之免疫反應

The Immuno-Responses of Eel (Anguilla Japonica) Inoculated with Edwardsiella Anguillimortiferum & Aeromonas Hydrophila Antigens

宋延齡(Yen-Ling Song) ； 郭光雄(Guang-Hsiung Kou)

《臺灣水產學會刊》 6卷1期 (1977/12) Pp. 65-72

https://doi.org/10.29822/JFST.197712.0008

摘要

養殖鰻於每年春季水溫劇變或夏季高水溫時，易感染潰瘍病(Edwardsiellosis)，造成重大損失。病原菌為Edwardsiella anguillimortiferum，此病之外觀症狀與Aeromonas hydrophila造成之赤鰭病類似，常共同感染。本實驗以主動免疫嘗試預防此二種症病，結果如下：
1．E. anguillimortiferum之加熱死菌與福馬林死菌抗原由腹腔注射免疫鰻魚後第2至第6星期抗體產生效價最高，達4096。利用菌浴法與腹腔注射再攻擊，免疫組之死亡率均較對照組低。
2．E. anguillimortiferum之加熱死菌與福馬林死菌皆誘使鰻產生抗體，但免疫性之長久維持以福馬林死菌較佳。
3．A. hydrophila與E. anguillimortiferum有部份抗原決定基團(antigenic determinats)相同。此相同部份對E. anguillimortiferum之抗體產生量有加强作用。
4．E. anguillimortiferum對鰻之抗原性(antigenecity)比A. hydrophila强。

並列摘要

Active immunization of the cultured eel was studied with Edwardsiella anguillimortiferum and Aeromonas hydrophila. They are pathogens of edwardsiellosis and red fin disease, respectively. The results were as following:
1. The immune response of eel was induced by intraperitoneal injection of heat or formalin-killed E. anguillimortiferum. The agglutinating titers reached their maxima (4096) two to six weeks after immunization. It seems that the immunized eels showed a higher resistant than the controlled ones.
2. The antibodies induced by the formalin-killed E. anguillimortiferum lasted longer than those induced by the heat-killed one.
3. Same antigenic determinants were common in both E. anguillimortiferum and A. hydrophila. Mixed injection of E. anguillimortiferum and A. hydrophila could enhance the agglutinating titer.
4. The antigenecity of E. anguillimortiferum was stronger than that of A. hydrophila.