不同氮源與鹽度對壇紫菜絲狀體生長之影響

Studies on the Effects of Nitrogen and Salinity on the Growth of the Conchocelis of Porphyra Haitanensis Chang and Zheng in Culture

江永棉(Young-Meng Chiang) ； 林秀華(Shio-Hua Lin)

《臺灣水產學會刊》 16卷3期 (1989/10) Pp. 189-195

https://doi.org/10.29822/JFST.198910.0004

摘要

以活體解剖取得牡蠣(Crassostrea gigas)卵。在室溫下，測試甘油、DMSO(dimethylsulfoxide)及propylene等冷凍保護劑對卵的毒性。藉trypan blue染色法判定卵的死活（活的不著色，死的呈藍色）。發現DMSO毒性最低。卵以10％的DMSO浸泡，10分鐘內置入Minicool AS 25冷凍儀（法國C.F.P.O.公司製），以5℃／min的速率由20℃降溫至-14℃，再以0.5℃/min的速率降至-30℃後，停留5分鐘，再以35℃/min降至-150℃之後，移入液態氮中保存。以水浴法(37℃)急速解凍。解凍卵加入新鮮精子使其受精。經10分鐘後以trypan blue染劑染色之。冷凍保存10分鐘者，活存率爲0.69±0.05(n=2)；保存1小時者活存率爲0.51±0.07（n=2）；保存4天者，活存率爲0.10±0.04（n=3）。

關鍵字

紫菜絲狀體 ； 紫菜培養 ； 紫菜屬 ； 壇紫菜

並列摘要

Oocytes of the Pacific oyster,Crassostrea gigas were obtained from theovaries of dissected oysters,incubated with cryoprotectants for 10min at 20℃and then stained with 0.5% trypan blue to investigate the toxicity of thecryoprotectants.Twenty per cent(V/V)of DMSO(dimethyl sulfoxide),propylene and glycerol caused 15,41 and 68% respectively of mortality of theoocyte.Oyster oocytes were incubated with 10% DMSO for 10min,frozenfrom 20℃ to -14℃ at a rate of 5℃/min,then to -30℃(0.5℃/min),holdingfor 5min,quickly to -150℃(35℃/min)and stored in LN2.After thawingwith 37℃ water bath,oocytes were fertilized with fresh sperm and stainedwith trypan blue 10min later.The survival ratio of oocytes stored in LN2for 10min,1hr and 4 days was 0.69±0.05(n=2),0.51±0.07(n=2)and 0.10±0.04(n=3),respectively.

並列關鍵字

Conchocelis ； Cultivation of Porphyra ； Porphyra Haitanesis