Testosterone-induced Changes in Protein Profiles of Seminal Vesicle and Testis in the Catfish, Clarias batrachus: A SDS-PAGE Study

雄性素誘導鯰魚儲精囊及精巢內的蛋白質含量產生變動：以SDS-PAGE分析

Madhu Shree Singh ； Keerikattil Paily Joy ； V. Samuel Raj ； Indrajeet Chow Dhury

《臺灣水產學會刊》 29卷2期 (2002/06) Pp. 107-116

https://doi.org/10.29822/JFST.200206.0002

摘要

實驗細分為三個小實驗，首先於準備期，注射雄性素1.0μg/g BW至鯰魚，每週一次，為期三週，緊接著在產卵前一週，劑量改為1.0或2.0μg/g BW，最後於非繁殖季節，注射或0.5 或1.0μg/g BW二週，其結果促使不同分子量蛋白質的合成。在第一個實驗（準備期）中，發現儲精囊在第一及二週有15.6及17.5KD的蛋白質出現，而於第一週亦出現35、41、46 及58KD的蛋白質；而在精巢，雄性素第一及二週可誘導18.2、32.5、175及190KD的蛋白質合成。第二個實驗（產卵前期），在SDS-PAGE分析後，發現在劑量為1.0μg/g BW的處理組之儲精囊有各式新蛋白質合成，其中以170及26KD的蛋白質最為顯著，但蛋白質合成的誘導效應在2.0μg/g BW的處理組，則較為低下。在第三個實驗（非繁殖期），為期二週的處理可強烈地誘導15.6及17.5KD的蛋白質的大量儲存。本研究結果顯示雄性素誘導鯰魚儲精囊及精巢內的蛋白質含量與種類的增加。

關鍵字

儲精囊 ； 精巢 ； 雄性素 ； 蛋白質 ； 35S-結合鯰魚 ； SDS-PAGE

並列摘要

In the catfish Clarias batrachus, administration of testosterone (1.0 μg/g BW for 1, 2 and 3 weeks in preparatory phase, 1.0 μg or 2.0 μg/g BW for 1 week in prespawning phase, and 0.5 μg or 1.0 μg/g BW for 2 weeks in resting phase) resulted in stimulation of protein synthesis as evident from appearance of a number of proteins of different molecular weights. In the first study (preparatory phase), resolution of 15.6 and 17.5 KD proteins in week 1 and 2, and 35, 41, 46 and 58 KD proteins in week 1 were noticed in the seminal vesicle (SV). In the testis, the treatment induced 18.2, 32.5, 175 and 190 KD proteins in the first and second weeks. In the second study (prespawning phase), induction of new proteins in the SV was monitored by 35S-methionine labeling of SDS-PAGE gel profiles. The f luorography of the gels showed a large number of new proteins that are not resolved in the control groups. 170 and 26 KD proteins were strongly labeled in the 1.0 μg testosterone treatment group. Besides, 136, 105, 94, 76, 60, 59, 53, 46, 40, 35 and 31 KD proteins showed weak to moderate labeling in the 1.0 μg testosterone group. The labeling was relatively weaker in 2.0 μg testosterone group in the seminal vesicle. In the third study (resting phase), 2 weeks of testosterone treatment resulted in resolution of 15.6 and 17.5 KD proteins, and 21.6 KD protein band was very thick suggesting high storage of this protein. The results of this study show that testosterone has stimulated accumulation of existing proteins or synthesis of new proteins in the testis or seminal vesicle.

並列關鍵字

Seminal vesicle ； Testis ； Testosterone ； Proteins ； 35S-incorporation Catfish ； SDS-PAGE